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Description of three bacterial strains belonging to the new genus Novipirellula gen. nov., reclassificiation of Rhodopirellula rosea and Rhodopirellula caenicola and readjustment of the genus threshold of the phylogenetic marker rpoB for Planctomycetaceae

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Abstract

Access to axenic cultures of Planctomycetes is crucial for further investigating their complex lifestyle, uncommon cell biology and primary and secondary metabolism. As a contribution to achieve this goal in the future, we here describe three strains belonging to the novel genus Novipirellula gen. nov. The strains were isolated from biotic and abiotic surfaces in the Baltic Sea and from the island Heligoland in the North Sea. Colony colours range from white to light pink. Cells are acorn-shaped and grew optimally at neutral pH and temperatures between 27 and 30 °C. Phylogenetic analyses revealed that the isolated strains represent three novel species belonging to a new genus, Novipirellula gen. nov. Beyond that, our analysis suggests that Rhodopirellula rosea LHWP3T, Rhodopirellula caenicola YM26-125T and Rhodopirellula maiorica SM1 are also members of this novel genus. Splitting the current genus Rhodopirellula into a more strictly defined genus Rhodopirellula and Novipirellula also allowed readjusting the genus threshold value for the gene rpoB, encoding the RNA polymerase β-subunit, which is used as phylogenetic marker for Planctomycetales. A threshold range of 75.5–78% identity of the analysed partial rpoB sequence turned out to be reliable for differentiation of genera within the family Planctomycetaceae.

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Acknowledgements

Part of this research was funded by the Deutsche Forschungsgemeinschaft Grants KA 4967/1-1 and JO 893/4-1, Grant ALWOP.308 of the Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO), SIAM (Soehngen Institute for Anaerobic Microbiology) Grant no. 024002002 and the Radboud Excellence fellowship. We thank Ina Schleicher for skillful technical assistance. We also thank our collaborators Sonja Oberbeckmann, Matthias Labrenz (IOW Warnemünde, Germany), Jörn Petersen (DSMZ) and the Biological Institute Heligoland (BAH) for sampling support. We thank Brian Tindall and Regine Fähnrich as well as the BCCM/LMG Bacteria collection for on-going support during strain deposition.

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  1. Department of Microbiology, Radboud Universiteit, Nijmegen, The Netherlands

    Nicolai Kallscheuer, Sandra Wiegand, Stijn H. Peeters, Mareike Jogler, Mike S. M. Jetten & Christian Jogler

  2. Leibniz Institute DSMZ, Brunswick, Germany

    Mareike Jogler, Christian Boedeker, Anja Heuer & Patrick Rast

  3. Central Facility for Microscopy, Helmholtz Centre for Infection Research, Brunswick, Germany

    Manfred Rohde

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  1. Nicolai Kallscheuer
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  2. Sandra Wiegand
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Contributions

NK and SW wrote the manuscript, analysed data and prepared figures, AH, PR and MJ isolated the strains and performed the initial cultivation and strain deposition, SHP and CB performed the light microscopic analysis, MSMJ contributed to text preparation and revised the manuscript, MR performed the electron microscopic analysis, CJ, PR and AH took the samples in the Baltic Sea and on Heligoland, and CJ supervised the study. All authors read and approved the final version of the manuscript.

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Correspondence to Christian Jogler.

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Kallscheuer, N., Wiegand, S., Peeters, S.H. et al. Description of three bacterial strains belonging to the new genus Novipirellula gen. nov., reclassificiation of Rhodopirellula rosea and Rhodopirellula caenicola and readjustment of the genus threshold of the phylogenetic marker rpoB for Planctomycetaceae. Antonie van Leeuwenhoek 113, 1779–1795 (2020). https://doi.org/10.1007/s10482-019-01374-5

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