Skip to main content
SpringerLink
Log in

Yeast communities of primary and secondary peat swamp forests in southern Thailand

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Khanthuli peat swamp forest (PSF) is one of a few fertile peat swamp forests that remain in Thailand. It is composed of primary PSF and some areas which have been degraded to secondary PSF due to drought, wildfires and land conversion, which have resulted in a decrease in peat layers and change in the species of the plant community. In this study, diversity of yeasts in peat from both primary and secondary PSF areas of the Khanthuli PSF was determined based on culture-dependent approaches, using dilution plate and enrichment techniques. A total of 66 yeast isolates were identified by the analysis of sequence similarity of the D1/D2 region of the large subunit rRNA gene or the combined analysis of sequence of the D1/D2 region and internal transcribed spacer region and confirmed by phylogenetic analysis of the D1/D2 region to belong to 22 known yeast species and six potential new species in the genera Candida (Kurtzmaniella, Lodderomyces, Ogataea, Pichia and Yamadazyma clades), Clavispora, Cyberlindnera, Galactomyces, Hanseniaspora, Metschnikowia, Saturnispora, Schwanniomyces, Cryptotrichosporon, Pichia, Curvibasidium, Papiliotrema, Rhodotorula, and Saitozyma. The most prevalent yeasts in the primary PSF were Cyberlindnera subsufficiens and Galactomyces candidus, while Saitozyma podzolica was the most frequently found in peat from the secondary PSF. Common yeast species in both, primary and secondary PSF, were Cy. subsufficiens, G. candidus and Rhodotorula mucilaginosa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Agus F, Hairiah K, Mulyani A (2011) Measuring carbon stock in peat soils: practical guidelines. World Agroforestry Centre (ICRAF) Southeast Asia Regional Program, Indonesian Centre for Agricultural Land Resources Research and Development, Bogor

    Google Scholar 

  • Ahearn DG, Roth FJ, Meyers SP (1968) Ecology and characterization of yeasts from aquatic regions of south Florida. Mar Biol 1:291–308

    Article  Google Scholar 

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Am-In S, Limtong S, Yongmanitchai W, Jindamorakot S (2011) Candida andamanensis sp. nov., Candida laemsonensis sp. nov. and Candida ranongensis sp. nov., anamorphic yeast species isolated from estuarine waters in a Thai mangrove forest. Int J Syst Evol Microbiol 61(Pt 2):454–461

    Article  CAS  PubMed  Google Scholar 

  • Andriesse JP (1988) Nature and management of tropical peat soils, 1st edn. FAO, Rome

    Google Scholar 

  • Birkhofer K, Schöning I, Alt F, Herold N, Klarner B, Maraun M, Schrumpf M (2012) General relationships between abiotic soil properties and soil biota across spatial scales and different land-use types. PLoS ONE 7:e43292

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Boonmak C, Jindamorakot S, Kawasaki H, Yongmanitchai W, Suwanarit P, Nakase T, Limtong S (2009) Candida siamensis sp. nov., an anamorphic yeast species in the Saturnispora clade isolated in Thailand. FEMS Yeast Res 9(4):668–672

    Article  CAS  PubMed  Google Scholar 

  • Boonyuen N, Sri-Indrasutdhi V, Suetrong S, Sivichai S, Jones EG (2012) Annulatascus aquatorba sp. nov., a lignicolous freshwater ascomycete from Sirindhorn Peat Swamp Forest, Narathiwat. Thailand Mycologia 104:746–757

    Article  PubMed  Google Scholar 

  • Brown S, Lugo AE (1990) Tropical secondary forests. J Trop Ecol 6(1):1–32

    Article  Google Scholar 

  • Buzzini P, Lachance MA, Yurkov A (2017) Yeasts in natural ecosystems: diversity. Springer, Switzerland

    Book  Google Scholar 

  • Colombo AL, Júnior JNA, Guinea J (2017) Emerging multidrug-resistant Candida species. Curr Opin Infect Dis 30(6):528–538

    Article  PubMed  Google Scholar 

  • Evers S, Yule CM, Padfield R, O'Reilly P, Varkkey H (2017) Keep wetlands wet: the myth of sustainable development of tropical peatlands—implications for policies and management. Global Change Biol 23(2):534–549

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  PubMed  Google Scholar 

  • Firdaus MS, Gandaseca S, Ahmed OH (2011) Effect of drainage and land clearing on selected peat soil physical properties of secondary peat swamp forest. Int J Phys Sci 6(23):5462–5466

    Google Scholar 

  • Ghazoul J, Sheil D (2010) Tropical rain forest ecology, diversity, and conservation. Oxford University Press Inc, New York

    Google Scholar 

  • Guinea J (2014) Global trends in the distribution of Candida species causing candidemia. Clin Microbiol Infect 20(6):5–10

    Article  PubMed  Google Scholar 

  • Hadi A, Fatah L, Affandi DN, Bakar RA, Inubushi K (2012) Population and genetic diversities of bacteria related to nitrous oxide and methane in peat soils of South Kalimantan, Indonesia. Malays J Soil Sci 16:121–135

    Google Scholar 

  • Hagler AN, Mendonça-Hagler LC, Pagnocca FC (2017) Yeasts in aquatic ecotone habitats. In: Buzzini P, Lachance MA, Yurkov A (eds) Yeasts in natural ecosystems: diversity. Springer, Switzerland, pp 70–74

    Google Scholar 

  • Hankaew MC (2000) Successional trend of peat swamp forests at Phru Toh Daeng and Phru Kuan Kreng, Southern Thailand. Dissertation, Kasetsart University

  • Jackson CR, Liew KC, Yule CM (2009) Structural and functional changes with depth in microbial communities in a tropical Malaysian peat swamp forest. Microb Ecol 57:402–412

    Article  PubMed  Google Scholar 

  • Jaiboon K, Lertwattanasakul N, Limtong P, Limtong S (2016) Yeasts from peat in a tropical peat swamp forest in Thailand and their ability to produce ethanol, indole-3-acetic acid and extracellular enzymes. Mycol Prog 15:755–770

    Article  Google Scholar 

  • John P, Sherry M, Bruce H, Jan J, John LK, Maurice E, Ann MW, Nancy W (2003) Recommended methods of manure analysis. University of Wisconsin–Madison, Madison

    Google Scholar 

  • Kachalkin AV, Yurkov AM (2012) Yeast communities in Sphagnum phyllosphere along the temperature-moisture ecocline in the boreal forest-swamp ecosystem and description of Candida sphagnicola sp. nov. Antonie Van Leeuwenhoek 102:29–43

    Article  PubMed  Google Scholar 

  • Kachalkin AV, Glushakova AM, Yurkov AM, Chernov IY (2008) Characterization of yeast groupings in the phyllosphere of Sphagnum mosses. Microbiology 77:474–481

    Article  CAS  Google Scholar 

  • Kaewwichian R, Yongmanitchai W, Srisuk N, Fujiyama K, Limtong S (2010) Geotrichum siamensis sp. nov. and Geotrichum phurueaensis sp. nov., two asexual arthroconidial yeast species isolated in Thailand. FEMS Yeast Res 10(2):214–220

    Article  CAS  Google Scholar 

  • Kaewwichian R, Khunnamwong P, Jindamorakot S, Lertwattanasakul N, Limtong S (2018) Cryptotrichosporon siamense sp. nov., a ballistoconidium-forming yeast species in Trichosporonales isolated in Thailand. Int J Syst Evol Microbiol 68:2473–2477

    Article  PubMed  CAS  Google Scholar 

  • Kanokratana P, Uengwetwanit T, Rattanachomsri U, Bunterngsook B, Nimchua T, Tangphatsornruang S, Plengvidhya V, Champreda V, Eurwilaichitr L (2011) Insights into the phylogeny and metabolic potential of a primary tropical peat swamp forest microbial community by metagenomic analysis. Microb Ecol 61:518–528

    Article  PubMed  Google Scholar 

  • Keddy PA (2010) Wetland ecology: principles and conversation, 2nd edn. The Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Khunnamwong P, Jindamorakot S, Limtong S (2018) Endophytic yeasts diversity in leaf tissue of rice, corn and sugarcane cultivated in Thailand assessed by a culture-dependent approach. Fungal Biol 122:785–799

    Article  PubMed  Google Scholar 

  • Khunnamwong P, Limtong S (2018) Saturnispora kantuleensis f.a., sp. nov., a novel yeast species isolated from peat in a tropical peat swamp forest in Thailand. Int J Syst Evol Microbiol 68:1160–1164

    Article  PubMed  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kurtzman CP (2011) The yeasts, a taxonomic study, 5th edn. Elsevier, London, pp 521–543

    Book  Google Scholar 

  • Limtong S, Kaewwichian R, Yongmanitchai W, Kawasaki H (2014) Diversity of culturable yeasts in phylloplane of sugarcane in Thailand and their capability to produce indole-3-acetic acid. World J Microbiol Biotechnol 30:1785–1796

    Article  CAS  PubMed  Google Scholar 

  • Longman KA, Jenik J (1990) Tropical forest and its environment, 2nd edn. Longman Singapore Publishers (PTE) Ltd, Singapore

    Google Scholar 

  • Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University Press, New York

    Google Scholar 

  • Nitiyon S, Khunnamwong P, Lertwattanasakul N, Limtong S (2018) Candida kantuleensis sp. nov., a d-xylose-fermenting yeast species isolated from peat in a tropical peat swamp forest. Int J Syst Evol Microbiol 68:2313–2318

    Article  CAS  PubMed  Google Scholar 

  • Nuyim T (2003) Manual on peat swamp forests rehabitation and planting in Thailand. Pikulthong Royal Development Study Centre National Park, Narathiwat

    Google Scholar 

  • Office of Environmental Policy and Planning (2002) Biodiversity in Khanthuli wetland. Ministry of Science and Technology, Bangkok

    Google Scholar 

  • Office of Natural Resources and Environmental Policy and Planning (2009) A survey of wetland status in Thailand: swamp. https://chm-thai.onep.go.th/chm/Inlandwater/data/peat%20survey/index.html. Accessed 7 Sept 2018

  • Page S, Rieley JO, Banks CJ (2011) Global and regional importance of the tropical peatland carbon pool. Global Change Biol 17:798–818

    Article  Google Scholar 

  • Pinnoi A, Lumyong S, Hyde KD, Jones EG (2006) Biodiversity of fungi on the palm Eleiodoxa conferta in Sirindhorn peat swamp forest, Narathiwat, Thailand. Fungal Divers 22:205–218

    Google Scholar 

  • Polburee P, Lertwattanasakul N, Limtong P, Groenewald M, Limtong S (2017) Nakazawaea todaengensis f.a., sp. nov., a yeast isolated from a peat swamp forest in Thailand. Int J Syst Evol Microbiol 67:2377–2382

    Article  CAS  PubMed  Google Scholar 

  • Polyakova AV, Chernov IY, Panikov NS (2001) Yeast diversity in hydromorphic soils with reference to a grass-Sphagnum wetland in western Siberia and a hummocky tundra region at Cape Barrow (Alaska). Microbiology 70:617–622

    Article  CAS  Google Scholar 

  • Posa MRC, Wijedasa LS, Corlett RT (2011) Biodiversity and conservation of tropical peat swamp forests. Bioscience 61:49–57

    Article  Google Scholar 

  • Rieley J, Page S (2016) Tropical peatland of the world. In: Osaki M, Tsuji N (eds) Tropical peatland ecosystems. Springer, Tokyo, pp 3–32

    Chapter  Google Scholar 

  • Sirirattanagon S (1994) Structural characteristics of To Daeng Peat Swamp Forest, Changwat Narathiwat. Dissertation, Kasetsart University

  • Sparks DL, Page AL, Helmke PA, Loeppert RH, Soluanpour PN, Tabatabai MA, Johnston CT, Sumner ME (1996) Method of soil analysis part 3: chemical method. Soil Science Society of America, Inc., American Society of Agronomy and Inc., Madison

    Book  Google Scholar 

  • Starmer WT, Lachance MA (2011) Yeast ecology. In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, London, pp 65–69

    Chapter  Google Scholar 

  • Stivrins N, Ozola I, Gałka M, Kuske E, Alliksaar T, Andersen TJ, Lamentowicz M, Wulf S, Reitalu T (2017) Drivers of peat accumulation rate in a raised bog: impact of drainage, climate, and local vegetation composition. Mires Peat 19(8):1–19

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • Thormann MN, Rice AV, Beilman DW (2007) Yeasts in peatlands: a review of richness and roles in peat decomposition. Wetlands 27:761–773

    Article  Google Scholar 

  • Tiner RW (1999) Wetland indicators: a guide to wetland identification, delineation, classification, and mapping. Lewis publishers, New York

    Book  Google Scholar 

  • Vijarnsorn P, Suzuki K, Kyuma K, Wada E, Nagano T, Takai Y (1995) A tropical swamp forest ecosystem and its greenhouse gas emission. Nodai Research Institute, Tokyo

    Google Scholar 

  • Walkley A, Black IA (1934) An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38

    Article  CAS  Google Scholar 

  • Wang H, Xu YC, Hsueh PR (2016) Epidemiology of candidemia and antifungal susceptibility in invasive Candida species in the Asia-Pacific region. Future Microbiol 11:1461–1477

    Article  CAS  PubMed  Google Scholar 

  • Whitmore TC (1975) Tropical rain forests of the Far East. Clarendon Press, Oxford

    Google Scholar 

  • Wösten JHM, Van Den Berg J, Van Eijk P, Gevers GJM, Giesen WBJT, Hooijer A, Idris A, Leenman PH, Rais DS, Siderius C, Silvius MJ, Suryadiputra N, Wibisono IT (2006) Interrelationships between hydrology and ecology in fire degraded tropical peat swamp forests. Water Resour Dev 22(1):157–174

    Article  Google Scholar 

  • Yurkov AM (2018) Yeasts of the soil: obscure but precious. Yeast 35(5):369–378

    Article  CAS  PubMed  Google Scholar 

  • Yurkov AM, Kemler M, Begerow D (2012) Assessment of yeast diversity in soils under different management regimes. Fungal Ecol 5(1):24–35

    Article  Google Scholar 

  • Zuza-Alves DL, Silva-Rocha WP, Chaves GM (2017) An update on Candida tropicalis based on basic and clinical approaches. Front Microbiol 8:1927

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This work was supported by the Center of Excellence on Biodiversity, Postgraduate Education and Research Development Office (PERDO) through the Project ‘Diversity of yeast in peat swamp forest and their potential in agricultural application’ Grant No. BDC-PG1-160001.

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Chanita Boonmak, Pannida Khunnamwong & Savitree Limtong

  2. Academy of Science, The Royal Society of Thailand, Bangkok, 10300, Thailand

    Savitree Limtong

Authors
  1. Chanita Boonmak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pannida Khunnamwong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Savitree Limtong
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

CB: Data analysis and wrote the manuscript. PK: Performed experiments and data analysis. SL: Designed study, data discussion and editing the manuscript.

Corresponding author

Correspondence to Savitree Limtong.

Ethics declarations

Conflicts of interest

The authors declare that there are no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 14 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Boonmak, C., Khunnamwong, P. & Limtong, S. Yeast communities of primary and secondary peat swamp forests in southern Thailand. Antonie van Leeuwenhoek 113, 55–69 (2020). https://doi.org/10.1007/s10482-019-01317-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-019-01317-0

Keywords

Search

Navigation