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Putative virulence factors of Plesiomonas shigelloides

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Abstract

Plesiomonas shigelloides is a Gram-negative rod-shaped bacterium which has been isolated from humans, animals and the environment. It has been associated with diarrhoeal disease in humans and various epizootic diseases in animals. In this study P. shigelloides strains were isolated from the faecal material of a captive Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis; YFP) living in semi-natural conditions in China. Plesiomonas shigelloides strain EE2 was subjected to whole genome sequencing. The draft genome was then compared to the genome sequences of ten other P. shigelloides isolates using the Pathosystems Resource Integration Center pipeline. In addition to several virulence factors which have been previously reported, we are proposing new candidate virulence factors such as a repeats-in-toxin protein, lysophospholipase, a twin-arginine translocation system and the type VI secretion effector Phospholipase A1.

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Acknowledgments

The authors thank Rebecca Wattam at Virginia Tech for her assistance on the use of PATRIC. This research was done as part of a Provost Honors project under the leadership of Zina Haywood, Executive Vice President/Provost. We thank Jennifer Cumpston and Donald Zakutansky for their enthusiastic support of this research. We also thank the staff of the Tian-e-Zhou National Natural Reserve for their help in collecting faecal samples. This work was supported by Grants from the Bureau of Science and Technology for Development, Chinese Academy of Sciences (No. ZSSD-004); the National Natural Science Foundation of China (Nos. 31430080, 31870372); and the Special Conservation Fund for the Yangtze finless porpoise from the Ministry of Agriculture of China.

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  1. General Studies, Gateway Technical College, Kenosha, WI, 53144, USA

    Melissa S. Edwards & Richard William McLaughlin

  2. The Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    Richard William McLaughlin, Jia Li, XiaoLing Wan, YuJiang Hao & JinSong Zheng

  3. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    Yingli Liu & HaiXia Xie

  4. University of Chinese Academy of Sciences, Beijing, 100039, China

    Jia Li, XiaoLing Wan & Yingli Liu

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  1. Melissa S. Edwards
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Edwards, M.S., McLaughlin, R.W., Li, J. et al. Putative virulence factors of Plesiomonas shigelloides. Antonie van Leeuwenhoek 112, 1815–1826 (2019). https://doi.org/10.1007/s10482-019-01303-6

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