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Ancylomarina psychrotolerans sp. nov., isolated from sediments of Fildes Peninsula and emended the description of genus Ancylomarina

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Abstract

A Gram-stain negative, obligately anaerobic, non-motile, asporogenous long rod-shaped and non-flagellated bacterial strain, designated 4SWWS2-6T, was isolated from sediment in the intertidal zone of Fildes Peninsula, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4SWWS2-6T belongs to the genus Ancylomarina and showed high sequence similarity with Ancylomarina subtilis FA102T (96.5%). Optimal growth occurred at pH 6.5, 16 °C and in the presence of 3% (w/v) NaCl. Strain 4SWWS2-6T contained menaquinone-7 (MK-7) as the major respiratory quinone and held iso-C15:0, anteiso-C15:0 and iso-C15:0 3-OH as the major cellular fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an aminolipid, two unidentified lipids and an unidentified phospholipid. The DNA G + C content of strain 4SWWS2-6T was 37.6 mol%. On the basis of the polyphasic analyses, strain 4SWWS2-6T is considered to represent a novel species in the genus Ancylomarina, for which the name Ancylomarina psychrotolerans sp. nov. is proposed. The type strain is 4SWWS2-6T (= KCTC 15504T = MCCC 1K01618T).

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Acknowledgements

We are thankful to the work of Prof. Aharon Oren from the Hebrew University of Jerusalem to name the strain and Prof. Zong-Jun Du from Shandong University to provide the reference strains. This work was supported by the Chinese Polar Environment Comprehensive Investigation and Assessment Program (No. CHINARE2016-02-01, CHINARE2016-01-06), projects from the National Natural Science Foundation of China (No. 41,476,112 and No. 41,521,064) and the National Key Research and Development Program of China (No. 2016YFA0601303).

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Author notes

  1. Chao Jia and Hong-chang Cui contributed equally to this work.

Authors and Affiliations

  1. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, People’s Republic of China

    Xiao-chong Shi & Xiao-Hua Zhang

  2. College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, 266003, People’s Republic of China

    Chao Jia, Hong-chang Cui, Yan-qiong Han, Tian-yu Fu, Rui Du, Xiao-lei Wang, Xiao-chong Shi & Xiao-Hua Zhang

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  1. Chao Jia
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  2. Hong-chang Cui
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  3. Yan-qiong Han
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  4. Tian-yu Fu
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  5. Rui Du
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Correspondence to Xiao-chong Shi.

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Additional information

The GenBank Accession Number for the 16S rRNA gene sequence of Ancylomarina psychrotolerans 4SWWS2-6T (=KCTC 15504T=MCCC 1K01618T) is KT266597.

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Jia, C., Cui, Hc., Han, Yq. et al. Ancylomarina psychrotolerans sp. nov., isolated from sediments of Fildes Peninsula and emended the description of genus Ancylomarina. Antonie van Leeuwenhoek 111, 1183–1189 (2018). https://doi.org/10.1007/s10482-018-1025-9

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