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Aquidulcibacter paucihalophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae isolated from cyanobacterial aggregates in a eutrophic lake

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Abstract

A bacterial strain, TH1-2T, was isolated from cyanobacterial aggregates in the eutrophic Lake Taihu, Jiangsu Province, China. Cells were observed to be Gram-negative, slightly curved and rod-shaped. Optimal growth was obtained at pH 7.0 (range 5.5–8.5) and 30 °C (range 20–37 °C) in trypticase soy broth (TSB) without NaCl. Growth was not observed in TSB with 1.0% (w/v) NaCl added. The cells were found to be positive for oxidase and catalase activities. The major fatty acids were identified as 3-hydroxy hexadecanoic acid (C16:0 3-OH), C16:1 ω5c and summed feature 8 (consisting of C18:1 ω6c and cis-11-Octadecenoic acid (C18:1 ω7c). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The 16S rRNA gene sequence of strain TH1-2T was found to be phylogenetically related to those of Glycocaulis abyssi MCS 33T and Brevundimonas naejangsanensis BIO-TAS2-2T (90.5 and 90.0% similarity, respectively). The genomic G+C content of strain TH1-2T was 55.6 mol% based on whole genome calculations. Average nucleotide identities (ANI) and the digital DNA–DNA hybridizations (DDH) for complete genomes ranged from 66.84  to 67.32  and 21.3 to 31.8% between strain TH1-2T and type strains within the family Caulobacteraceae, higher than those between strain TH1-2T and the strains within the family Hyphomonadaceae. The phenotypic, chemotaxonomic and phylogenetic properties, and genome analysis, indicate that strain TH1-2T (=CGMCC 1.12979T = LMG 28362T) represents a novel species in a new genus within the family Caulobacteraceae; thus, the name Aquidulcibacter paucihalophilus gen. nov., sp. nov., is proposed.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 31100021 and 31100582), Natural Science Foundation of Jiangsu Province of China (BK20151612) and open research fund of State Key Laboratory of Marine Geology, Tongji University, China (No. MGK1605). We thank Dr Yonghui Zeng (Aarhus University, Denmark) for financial assistance for the genome sequencing of strain TH1-2T. We thank Prof. Lee R. Krumholz for assistance with English.

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Authors and Affiliations

  1. State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, China

    Haiyuan Cai, Yuan Shi & Helong Jiang

  2. Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou, China

    Yanan Wang

  3. School of Food & Biological Engineering, Jiangsu University, Zhenjiang, China

    Henglin Cui

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  1. Haiyuan Cai
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  2. Yuan Shi
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Correspondence to Helong Jiang.

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Cai, H., Shi, Y., Wang, Y. et al. Aquidulcibacter paucihalophilus gen. nov., sp. nov., a novel member of family Caulobacteraceae isolated from cyanobacterial aggregates in a eutrophic lake. Antonie van Leeuwenhoek 110, 1169–1177 (2017). https://doi.org/10.1007/s10482-017-0889-4

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