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Skermanella mucosa sp. nov., isolated from crude oil contaminated soil

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Abstract

A novel Gram-stain negative, small rod-shaped bacterium (strain 8-14-6T) was isolated from hydrocarbon contaminated desert soil collected from Kuwait. Strain 8-14-6T grew at 5–37 °C, pH 6.0–8.8 and 0–2% (w/v) of NaCl concentration. Casein, starch, Tween 20 and Tween 80 were hydrolyzed while urea, chitin, DNA and carboxymethyl-cellulose were not hydrolyzed by strain 8-14-6T. The major cellular fatty acids were identified as C18:1ω6c/C18:1ω7c, C16:0 and iso-C16:1I/C14:03-OH. Strain 8-14-6T produced diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipids, two unidentified lipids and five unidentified amino lipids as polar lipids. Genomic G+C content was 73.5 mol %. 16S rRNA gene sequence comparisons indicated that strain 8-14-6T represents a member of the genus Skermanella within family Rhodospirillaceae of the class Alphaproteobacteria. Strain 8-14-6T has a sequence similarity of 98.9% with Skermanella rosea M1T, 97.4% with Skermanella aerolata 5416T-32T, 96.9% with Skermanella stibiiresistens SB22T and <95.4% with the other two known species of the genus Skermanella. The DNA–DNA relatedness values between strain 8-14-6T and the type strains of the closely related species were clearly below the 70% threshold. From the combination of phenotypic and genotypic characteristics and distinct phylogenetic position, the strain is considered to represent a novel species of the genus Skermanella, for which the name Skermanella mucosa sp. nov. is proposed. The type strain is 8-14-6T (=KEMB 2255-438T =JCM 31590T).

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Acknowledgements

National Instrumentation Center for Environmental Management (NICEM), Seoul National University, South Korea is acknowledged for helping us in the Transmission Electron Microscopy.

Funding

This research was supported by a Grant (16SCIP-B103706-02) from Construction Technology Research Program funded by Ministry of Land, Infrastructure and Transport of Korean government. Authors also acknowledge Korea Environmental Microorganism Bank (NRF-2015M3A9B8029697) for providing the funding support. Ministry of Science, ICT and future planning of Korean Government is greatly acknowledged for providing the funding support (NRF-2013M3A9A5076601).

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Authors and Affiliations

  1. Department of Life Science, College of Natural Science, Kyonggi University, 94-6 Iui-dong Yeongtong-gu, Suwon, 443-760, Republic of Korea

    Y. Subhash, Da-Eun Yoon & Sang-Seob Lee

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  1. Y. Subhash
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  2. Da-Eun Yoon
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  3. Sang-Seob Lee
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Correspondence to Sang-Seob Lee.

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The authors declare that there are no conflicts of interest.

Additional information

The EMBL accession numbers for the 16S rRNA gene sequence of strain 8-14-6T is LT158289.

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Subhash, Y., Yoon, DE. & Lee, SS. Skermanella mucosa sp. nov., isolated from crude oil contaminated soil. Antonie van Leeuwenhoek 110, 1053–1060 (2017). https://doi.org/10.1007/s10482-017-0878-7

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