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Rhizobium hedysari sp. nov., a novel species isolated from a root nodule of Hedysarum multijugum in China

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Abstract

A strain 5-1-2T was isolated from a root nodule of Hedysarum multijugum collected from Zhangye city, Gansu province, north-west China. Phylogenetic analysis based on the 16S rRNA gene sequence and other housekeeping genes (recA and atpD) indicated that the strain represents a novel species in the genus Rhizobium close to the strain Rhizobium subbaraonis JC85T with similarities of 98.27, 88.92 and 89.62%, respectively. Strain 5-1-2T contained Q-10 as the predominant ubiquinone. Our results showed that the major fatty acids were feature 8 (C18:1 ω7c and/or C18:1 ω6c; 38.90%). In addition, the DNA–DNA hybridizations with the type strains R. subbaraonis JC85T and Rhizobium halophytocola YC6881T were 39.2 ± 2.1 and 44.3 ± 1.9, respectively. Therefore, a novel species Rhizobium hedysari sp. nov. is proposed, and 5-1-2T (=CGMCC1.15677T = NBRC112532T) is designated as the type strain.

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Acknowledgements

This work was supported by projects from National Science Foundation of China (31360004), China Scholarship Council fund and Education Department of Gansu Province (2014A-107). We would like to express our gratitude to Professor Shuangjiang Liu and Chengying Jiang (Institute of Microbiology, Chinese Academy of Sciences, Beijing, China) for providing the type strains. Also we are grateful to professor J Peter W Young, Dr. Yong Zhang and Dr. Osama Abdalla Mohamad for revising our manuscript.

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Authors and Affiliations

  1. Key Laboratory of Western China’s Resources and Environment Chemistry, Hexi University, Zhangye, 734000, Gansu, China

    Lin Xu

  2. Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China

    Lin Xu, Jianfeng Shi, Caixia Li, Shengan Zhu & Bo Li

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  1. Lin Xu
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  2. Jianfeng Shi
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Correspondence to Lin Xu.

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Xu, L., Shi, J., Li, C. et al. Rhizobium hedysari sp. nov., a novel species isolated from a root nodule of Hedysarum multijugum in China. Antonie van Leeuwenhoek 110, 479–488 (2017). https://doi.org/10.1007/s10482-016-0817-z

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