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Kiloniella litopenaei sp. nov., isolated from the gut microflora of Pacific white shrimp, Litopenaeus vannamei

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Abstract

A cream-coloured rod-shaped bacterium, designated strain P1-1T, was isolated from the gut microflora of Pacific white shrimp (Litopenaeus vannamei) from the Taiwan Strait, China. P1-1T was was found to be Gram-negative, oxidase and catalase positive and motile by means of a single flagellum. Growth is observed at salinities of 0.5–7 % (w/v; optimum 3 %), at pH 6.0–10.0 (optimum pH 7.0–9.0) and at 15–37 °C (optimum 28–32 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P1-1T belongs to the genus Kiloniella, and is closely related to Kiloniella spongiae MEBiC09566T (99.0 % similarity), followed by Kiloniella laminariae LD81T (96.6 % similarity). The genomic ANIm value between strain P1-1T and K. spongiae MEBiC09566T was found to be 81.7 %. The DNA–DNA hybridization estimate between strains P1-1T and MEBiC09566T was 24.6 %. The principal fatty acids detected were identified as summed feature 8 (C18:1 ω6c/C18:1 ω7c, 74.2 %) and summed feature 3 (C16:1 ω6c/C16:1 ω7c, 9.7 %). The G+C content of the chromosomal DNA, determined from the draft genome sequence, was found to be 46.1 mol%. The major respiratory quinone was determined to be Q-9, while the major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, one unidentified aminolipid and nine unidentified lipids. The combined genotypic and phenotypic data indicate that strain P1-1T represents a novel species within the genus Kiloniella, for which the name Kiloniella litopenaei sp. nov. is proposed, with the type strain P1-1T (=MCCC 1A09869T = LMG 27755T).

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Acknowledgments

We are very grateful to Dr. Kae Kyoung Kwon, Marine & Extreme Bioresources Center, Korea Institute of Ocean Science & Technology, Republic of Korea, who provided the type strain Kiloniella spongiae MEBiC09566T. This work was financially supported by the project of Xiamen Southern Oceanographic Center (14CZP034HJ08), Public Welfare Project of SOA (201005032), and National Infrastructure of Microbial Resources of China (NIMR-2014-9 and NIMR-2015-9).

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Authors and Affiliations

  1. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150000, China

    Liping Wang & Zongze Shao

  2. State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China

    Liping Wang, Xiaoyi Li, Qiliang Lai & Zongze Shao

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  1. Liping Wang
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  2. Xiaoyi Li
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Correspondence to Zongze Shao.

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Wang, L., Li, X., Lai, Q. et al. Kiloniella litopenaei sp. nov., isolated from the gut microflora of Pacific white shrimp, Litopenaeus vannamei . Antonie van Leeuwenhoek 108, 1293–1299 (2015). https://doi.org/10.1007/s10482-015-0581-5

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