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A century later: rediscovery, culturing and phylogenetic analysis of Diploöspora rosea, a rare onygenalean hyphomycete

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Abstract

Nearly 100 years after its first discovery, Diploöspora rosea was detected on biologically damaged parchment paper in Rome, Italy and isolated from house dust collected in Micronesia. The isolation of this culture permitted morphological study of colony characters, conidium and conidiophore development, and phylogenetic investigations using sequences of nuc 18S rDNA, internal transcribed spacers, and 28S rDNA. The results indicate that D. rosea is an onygenalean fungus, of uncertain taxonomic position, basal or sister to the Gymnoascaceae. Based on observations of the parchments using SEM-Energy Dispersive Spectroscopy, we speculate that the fungus occurs in archival and domestic environments subject to periodic wetting. Its ability to grow on all low water activity media used in the study, including malt extract agar amended with 60 % sucrose, confirms its xerophilic nature.

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Acknowledgments

We thank Wayne Law and Carlos Cianchini for obtaining the Micronesian dust samples used in this study, CBS-KNAW for providing the D. longispora var. cubensis strain and Lynne Sigler for providing the UAMH D. longispora strains and for thought-provoking discussions. F. Pinzari is grateful to Dr. Dario Lunghini whose experience and knowledge was crucial for the identification of D. rosea in the parchment samples. We thank, Dr. Cobus Visagie for sharing his opinion on the identity of the Aspergillus on the type species of D. rosea. This research was supported by grants from the A. P. Sloan Foundation programme on the Microbiology of the Built Environment.

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Authors and Affiliations

  1. Biodiversity (Mycology and Microbiology), Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada

    Joey B. Tanney, Hai D. T. Nguyen & Keith A. Seifert

  2. Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria (CREA), Rome, Italy

    Flavia Pinzari

  3. Department of Life Sciences, Natural History Museum, London, UK

    Flavia Pinzari

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  1. Joey B. Tanney
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  2. Hai D. T. Nguyen
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  3. Flavia Pinzari
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  4. Keith A. Seifert
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Correspondence to Joey B. Tanney.

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Tanney, J.B., Nguyen, H.D.T., Pinzari, F. et al. A century later: rediscovery, culturing and phylogenetic analysis of Diploöspora rosea, a rare onygenalean hyphomycete. Antonie van Leeuwenhoek 108, 1023–1035 (2015). https://doi.org/10.1007/s10482-015-0555-7

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