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Planococcus dechangensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soils in Dechang Township, Zhaodong City, China

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Abstract

Strain NEAU-ST10-9T is a moderately halophilic, coccoid and non-motile bacterium isolated from saline and alkaline soils in the Dechang Township, Zhaodong City, China. The bacterium was found to be aerobic and Gram-stain positive. It forms orange colonies and grows at NaCl concentrations of 2–10 % (w/v) (optimum, 4 % w/v), at 4–50 °C (optimum, 30 °C) and at pH 6.0–10.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences indicated that it belongs to the genus Planococcus within the family Planococcaceae. The most closely related species was Planococcus maritimus, whose type strain (TF-9T) showed gene sequence similarities of 99.1 % for 16S rRNA, 83.7 % for gyrB and 87.0 % for rpoB with those of strain NEAU-ST10-9T, respectively. DNA–DNA hybridization relatedness values between strain NEAU-ST10-9T and type strains P. maritimus DSM 17275T , P. rifietoensis DSM 15069T , P. plakortidis DSM 23997T, P. citreus DSM 20549T, P. maitriensis DSM 15305T, P. salinarum KCTC 13584T and P. columbae DSM 17517T were from 55 ± 1 to 32 ± 2 %. The DNA G+C content was found to be 45.2 mol %. The major fatty acids (>5 %) were determined as C15:0 anteiso, C16:1 ω7c alcohol, C17:1 ω9c and C17:0 anteiso. The major menaquinones of strain NEAU-ST10-9T were identified as MK-7 and MK-8. The polar lipids were found to contain of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphocholine and two unknown lipids. The genotypic, chemotaxonomic and phenotypic analysis indicated that strain NEAU-ST10-9T represents a novel species of the genus Planococcus, for which we proposed the name Planococcus dechangensis sp. nov. The type strain is NEAU-ST10-9T (=CGMCC 1.12151T=DSM 25871T).

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 31370087), Fund for Fostering Talents in Basic Science of the National Natural Science Foundation of China (Grant No. J1210069), Natural Science Foundation of Heilongjiang Province of China (Grant No. C201417) and Postdoctoral Scientific Research Start-up Fund of Heilongjiang Province of China (Grant No. LBH-Q14022).

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    Authors and Affiliations

    1. Department of Microbiology and Biotechnology, College of Life Sciences, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

      Kaibiao Wang, Lei Zhang, Jianying Li, Yuanyuan Pan, Lin Meng, Tong Xu, Cheng Zhang, Henan Liu, Shan Hong, Haipeng Huang & Juquan Jiang

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    1. Kaibiao Wang
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    Correspondence to Juquan Jiang.

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    Kaibiao Wang and Lei Zhang have contributed equally to this work.

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    Wang, K., Zhang, L., Li, J. et al. Planococcus dechangensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soils in Dechang Township, Zhaodong City, China. Antonie van Leeuwenhoek 107, 1075–1083 (2015). https://doi.org/10.1007/s10482-015-0399-1

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