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Chryseobacterium polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Chryseobacterium

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Abstract

A Gram-stain negative, rod-shaped and non-endospore forming bacterium, designated strain YG4-6T, was isolated from Polytrichastrum formosum collected from Gawalong glacier in Tibet, China and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of strain YG4-6T were identified as iso-C15:0 (29.3 %), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c as defined by MIDI, 23.5 %) and iso-C17:0 3-OH (16.5 %). The major polar lipids were found to consist of five unidentified aminolipids and three unidentified lipids. Strain YG4-6T was found to contain MK-6 as the dominant menaquinone and the G+C content of its genomic DNA was determined to be 37.3 mol%. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YG4-6T is affiliated to Chryseobacterium species, and its closest related species were Chryseobacterium aahli T68T (97.9 % sequence similarity), Chryseobacterium zeae JM-1085T (97.8 % sequence similarity), Chryseobacterium yeoncheonense DCY67T (97.6 % sequence similarity) and Chryseobacterium soldanellicola NBRC 100864T (97.2 % sequence similarity). However, the DNA–DNA relatedness values between these strains and strain YG4-6T were found to be clearly below 70 %. Based on the phylogenetic inference and phenotypic data, strain YG4-6T is considered to represent a novel species of the genus Chryseobacterium, for which the name Chryseobacterium polytrichastri sp. nov. is proposed. The type strain is YG4-6T (=CGMCC 1.12491T = DSM 26899T). An emended description of the genus Chryseobacterium is also proposed.

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Acknowledgment

This work was funded by the Scientific Research Program of National Natural Science Foundation of China (No. 31100004 and No. 31470136).

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Authors and Affiliations

  1. College of Life Science, Capital Normal University, Xisanhuan North Road No. 105, Haidian District, Beijing, 100048, China

    Xin Yao Chen, Ran Zhao, Zhi Ling Chen, Lei Liu, Xue Dong Li & Yan Hong Li

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  1. Xin Yao Chen
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  2. Ran Zhao
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  3. Zhi Ling Chen
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  4. Lei Liu
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  5. Xue Dong Li
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Correspondence to Xue Dong Li or Yan Hong Li.

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10482_2014_338_MOESM1_ESM.docx

The phylogenetic trees made by neighbour-joining and maximum parsimony methods, transmission electron micrographs and the TLC of the total polar lipids of strain YG4-6T are available as supplementary figures with the online version of this paper. (DOCX 1697 kb)

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Chen, X.Y., Zhao, R., Chen, Z.L. et al. Chryseobacterium polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum), and emended description of the genus Chryseobacterium . Antonie van Leeuwenhoek 107, 403–410 (2015). https://doi.org/10.1007/s10482-014-0338-6

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