Skip to main content
SpringerLink
Log in

Litoribrevibacter albus gen. nov. sp. nov., isolated from coastal seawater, Fujian province, China

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-stain negative, short rod-shaped aerobic bacterium with flagella, designated strain Y32T, was isolated from coastal seawater in Xiamen, Fujian Province of China. 16S rRNA gene sequence comparisons showed that strain Y32T is a member of the family Oceanospirillaceae, forming a distinct lineage with species of the genus Litoribacillus. The 16S rRNA gene sequence similarities between strain Y32T and other strains were all less than 94.0 %. Strain Y32T was found to grow optimally at 28 °C, at pH 7.0–8.0 and in the presence of 4–5 % (w/v) NaCl. The major fatty acids were identified as Summed Feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c, 49.4 %), C16:0 (17.7 %), C14:0 (6.9 %) and C18:1 ω9c (5.4 %). The major respiratory quinone was identified as ubiquinone-8 (Q-8). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain Y32T was determined to be 55.6 mol%. According to its morphology, physiology, fatty acid composition, polar lipids composition and 16S rRNA gene sequence data, strain Y32T represents a novel species of a new genus in the family Oceanospirillaceae, for which the name Litoribrevibacter albus gen. nov. sp. nov. is proposed. The type strain of Litoribrevibacter albus is Y32T (=MCCC 1F01211T=NBRC 110071T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Abbreviations

MCCC:

Marine culture collection of China

NBRC:

Biological resource center national institute of technology and evaluation

CICC:

China center of industrial culture collection

References

  • Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1995) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology, 3rd edn. Wiley, New York

    Google Scholar 

  • Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070

    Article  PubMed  CAS  Google Scholar 

  • Bowman JP (2000) Description of Cellulophaga algicola sp. Nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868

    PubMed  CAS  Google Scholar 

  • Choi A, Cho JC (2010) Reinekea aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 60:2813–2817

    Article  PubMed  CAS  Google Scholar 

  • Choi A, Cho JC (2013) Thalassolituus marinus sp. nov., a hydrocarbonutilizing marine bacterium. Int J Syst Evol Microbiol 63:2234–2238

    Article  PubMed  CAS  Google Scholar 

  • Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  PubMed  CAS  Google Scholar 

  • Garrity GM, Bell JA, Lilburn T (2005) Family I. Oceanospirillaceae fam. nov. Bergey’s Manual of Systematic Bacteriology 2: 271

  • Hylemon P, Wells J, Krieg N, Jannasch H (1973) The genus Spirillum: a taxonomic study. Int J Syst Evol Microbiol 23:340–380

    CAS  Google Scholar 

  • Kates M (1986) Techniques of lipidology. 2nd ed. rev., Elsevier, Amsterdam, p 106–107, 241–246

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  PubMed  CAS  Google Scholar 

  • Krishna KK, Bhumika V, Thomas M, Anil Kumar P, Srinivas TNR (2013) Oceanospirillum nioense sp. nov., a marine bacterium isolated from sediment sample of Palk bay. India. Antonie van Leeuwenhoek 103:1015–1021

    Article  Google Scholar 

  • Li Y, Bai S, Yang C, Lai Q, Zhang H, Chen Z, Wei J, Zheng W, Tian Y, Zheng T (2013a) Mangrovimonas yunxiaonensis gen. nov., sp. nov., isolated from mangrove sediment. Int J Syst Evol Microbiol 63:2043–2048

    Article  PubMed  CAS  Google Scholar 

  • Li Y, Wei J, Yang C, Lai Q, Chen Z, Li D, Zhang H, Tian Y, Zheng W, Zheng T (2013b) Tenacibaculum xiamenense sp. nov., an algicidal species isolated from coastal seawater. Int J Syst Evol Microbiol 63:3481–3486

    Article  PubMed  CAS  Google Scholar 

  • Pinhassi J, Pujalte MJ, Pascual J, González JM, Lekunberri I, Pedrós-Alió C, Arahal DR (2009) Bermanella marisrubri gen. nov., sp. nov., a genome-sequenced gammaproteobacterium from the Red Sea. Int J Syst Evol Microbiol 59:373–377

    Article  PubMed  CAS  Google Scholar 

  • Rzhetsky A, Nei M (1993) Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095

    PubMed  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Teramoto M, Ohuchi M, Hatmanti A, Darmayati Y, Widyastuti Y, Harayama S, Fukunaga Y (2011) Oleibacter marinus gen. nov., sp. nov., a bacterium that degrades petroleum aliphatic hydrocarbons in a tropical marine environment. Int J Syst Evol Microbiol 61:375–380

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Yu M, Austin B, Zhang XH (2012) Oleispira lenta sp. nov., a novel marine bacterium isolated from Yellow sea coastal seawater in Qingdao. China. Antonie van Leeuwenhoek 101:787–794

    Article  Google Scholar 

  • Zhao R, Ji S, Wang Y, Yu T, Li Z, Li B, Shi X, Zhang XH (2013) Litoribacillus peritrichatus gen. nov. sp. nov., isolated from coastal sediment of an amphioxus breeding zone in Qingdao. China. Antonie van Leeuwenhoek 103:357–366

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Public Science and Technology Research Funds Projects of Ocean (201305016, 201305022), National Nature Science Foundation of China (40930847, 41376119). We would like to thank Professor I. J. Hodgkiss from Hong Kong University for help with English.

Author information

Authors and Affiliations

  1. Key Lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life Sciences, Xiamen University, Xiamen, 361005, People’s Republic of China

    Yi Li, Hong Zhu, Xueqian Lei, Huajun Zhang, Zhangran Chen, Wei Zheng, Yun Tian, Hong Xu & Tianling Zheng

  2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, People’s Republic of China

    Qiliang Lai

Authors
  1. Yi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hong Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qiliang Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xueqian Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Huajun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhangran Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Wei Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yun Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Hong Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Tianling Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Hong Xu or Tianling Zheng.

Electronic supplementary material

Below is the link to the electronic supplementary material.

10482_2014_260_MOESM1_ESM.docx

Transmission electron micrographs of cells of strain Y32T, polar lipids of strain Y32T, Maximum-Likelihood tree and Minimum Evolution tree are available as supplementary figures in ANTO Online. Supplementary material 1 (DOCX 2071 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, Y., Zhu, H., Lai, Q. et al. Litoribrevibacter albus gen. nov. sp. nov., isolated from coastal seawater, Fujian province, China. Antonie van Leeuwenhoek 106, 911–918 (2014). https://doi.org/10.1007/s10482-014-0260-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-014-0260-y

Keywords

Search

Navigation