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Roseovarius albus sp. nov., a new Alphaproteobacterium isolated from the Mediterranean Sea

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Abstract

Strain 4SM10T, an aerobic marine, Gram-negative, heterotrophic and non pigmented bacterium isolated from seawater from Vinaroz in Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence placed the strain within the Roseobacter clade in the family Rhodobacteraceae. Phylogenetic analyses also showed that strain 4SM10T forms a stable clade with species of the genus Roseovarius, being related to Roseovarius nubinhibens ISMT and Roseovarius aestuarii SMK-122T at 97.5 and 97.4 % 16S rRNA sequence similarity, respectively. Average Nucleotide Identity (ANI) values, determined as a measure of overall genomic resemblance, confirmed that strain 4SM10T does not belong to the same species as R. aestuarii CECT 7745T and Roseovarius nubinhibens CECT 7750T displaying ANI values well below the 95 % boundary for genomic species. Strain 4SM10T requires Na+ plus a divalent cation (either Mg2+ or Ca2+) to grow, reduces nitrate to nitrite and uses a large number of amino acids and organic acids (but no carbohydrates) as sole carbon sources. Enzymatic activities displayed in API ZYM tests are alkaline phosphatase, leucine arylamidase and acid phosphatase. The major cellular fatty acids were identified as C18:1 ω7c and/or C18:1 ω6c (67.1 %). The DNA G+C content was determined to be 54.27 mol%. Based on the genotypic and phenotypic data obtained, the name Roseovarius albus sp. nov. is proposed for this novel taxon, with the type strain 4SM10T (=CECT 7450T = KCTC 22653T).

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Abbreviations

ANI:

Average Nucleotide Identity

ASW:

Artificial sea water

DDH:

DNA–DNA hybridization

MA:

Marine agar

MCS:

Marine cations supplement

STA:

Salt tolerance agar

NJ:

Neighbor joining

MP:

Maximum parsimony

ML:

Maximun likelihood

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Acknowledgments

The study has been funded through projects CGL2010-18132/BOS (Spanish Ministerio de Economía y Competitividad) to MJP and PROMETEO2010-040 (Generalitat Valenciana) to Esperanza Garay.

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Authors and Affiliations

  1. Colección Española de Cultivos Tipo (CECT) and Departamento de Microbiología y Ecología, Universitat de València, Valencia, Spain

    Teresa Lucena, María A. Ruvira, M. Carmen Macián, María J. Pujalte & David R. Arahal

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  1. Teresa Lucena
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  2. María A. Ruvira
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  3. M. Carmen Macián
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  4. María J. Pujalte
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  5. David R. Arahal
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Correspondence to Teresa Lucena.

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Lucena, T., Ruvira, M.A., Macián, M.C. et al. Roseovarius albus sp. nov., a new Alphaproteobacterium isolated from the Mediterranean Sea. Antonie van Leeuwenhoek 105, 671–678 (2014). https://doi.org/10.1007/s10482-014-0121-8

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  • DOI: https://doi.org/10.1007/s10482-014-0121-8

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