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Tenacibaculum halocynthiae sp. nov., a member of the family Flavobacteriaceae isolated from sea squirt Halocynthia roretzi

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Abstract

A Gram-negative, non-spore-forming, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated P-R2A1-2T, was isolated from sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. It grew optimally at 25–28 °C, at pH 7.0–8.0 and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that the strain fell within the clade comprising Tenacibaculum species. Strain P-R2A1-2T exhibited the highest 16S rRNA gene sequence similarity values of 97.6, 97.2 and 97.0 % to Tenacibaculum aestuarii SMK-4T, T. lutimaris TF-26T and T. aiptasiae a4T, respectively, and of 94.5–96.8 % to the type strains of the other Tenacibaculum species. Strain P-R2A1-2T contained MK-6 as the predominant menaquinone and C16:1 ω7c and/or iso-C15:0 2-OH, iso-C15:0 3-OH and iso-C15:0 as the major fatty acids. The DNA G + C content of strain P-R2A1-2T was 30.7 mol % and its DNA–DNA relatedness values with the type strains of T. aestuarii, T. lutimaris and T. aiptasiae were 17 ± 4.2, 21 ± 6.1 and 16 ± 5.2 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separate from other Tenacibaculum species. On the basis of the data presented, strain P-R2A1-2T is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum halocynthiae sp. nov. is proposed. The type strain is P-R2A1-2T (=KCTC 32262T = CCUG 63681T).

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Acknowledgments

This work was supported by grants from the National Fisheries Research and Development Institute (NFRDI), Program for Collection, Management and Utilization of Biological Resources and BK21 program from the Ministry of Education, Science & Technology (MEST) of the Republic of Korea.

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Authors and Affiliations

  1. Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Busan, Gijang, 619-705, South Korea

    Young-Ok Kim, Bo-Hye Nam, Dong-Gyun Kim & Young-Ju Jee

  2. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Yong-Taek Jung & Jung-Hoon Yoon

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  1. Young-Ok Kim
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  2. Sooyeon Park
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  3. Bo-Hye Nam
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  7. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain P-R2A1-2T is JX912707.

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Kim, YO., Park, S., Nam, BH. et al. Tenacibaculum halocynthiae sp. nov., a member of the family Flavobacteriaceae isolated from sea squirt Halocynthia roretzi . Antonie van Leeuwenhoek 103, 1321–1327 (2013). https://doi.org/10.1007/s10482-013-9913-5

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