Skip to main content
SpringerLink
Log in

Marinobacter nanhaiticus sp. nov., polycyclic aromatic hydrocarbon-degrading bacterium isolated from the sediment of the South China Sea

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-negative, rod-shaped, slightly halophilic and facultatively anaerobic bacterium, designated strain D15-8WT, was isolated from the sediment of the South China Sea. Growth was found to occur optimally at 25 °C, between pH 7.0 and 8.0 and with 1–5 % (w/v) NaCl. The strain was observed to utilize a variety of organic substrates and polycyclic aromatic hydrocarbons as sole carbon sources. The G+C content of the genomic DNA was determined to be 58.7 %. The predominant respiratory quinone was found to be Q-9. The significant fatty acids were determined to be C16:0, C16:1 ω9c, C18:1 ω9c, C12:0 and C14:0 3OH. Analysis of 16S rRNA gene sequences showed that strain D15-8WT fits within the phylogenetic cluster of the genus Marinobacter and is most closely related to Marinobacter segnicrescens CGMCC 1.6489T, Marinobacter bryozoorum DSM 15401T, Marinobacter lacisalsi CECT 7297T and Marinobacter daqiaonensis CGMCC1.9167T. The DNA–DNA hybridization values between strain D15-8WT and the type strains of the most closely related species were 42.3 % (CGMCC 1.6489T), 39.8 % (DSM 15401T), 37.3 % (CECT 7297T) and 35.2 % (CGMCC1.9167T). The results of this polyphasic study indicate that strain D15-8WT represents a novel species of the genus Marinobacter, for which the name Marinobacter nanhaiticus sp. nov. is proposed. The type strain is D15-8WT (=CGMCC 1.11019T=KCTC 23749T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Aguilera M, Jiménez-Pranteda ML, Kharroub K, González-Paredes A, Durban JJ, Russell NJ, Ramos-Cormenzana A, Monteoliva-Sánchez M (2009) Marinobacter lacisalsi sp. nov., a moderately halophilic bacterium isolated from the saline-wetland wildfowl reserve Fuente de Piedra in southern Spain. Int J Syst Evol Microbiol 59:1691–1695

    Article  PubMed  CAS  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  • Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1995) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology, 3rd edn. Wiley, New York

    Google Scholar 

  • Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  PubMed  CAS  Google Scholar 

  • Collins MD (1985) Isoprenoid quinone analysis in bacterial classification and identification. In: Goodfellow M, Minnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 267–287

    Google Scholar 

  • Cui ZS, Shao ZZ (2009) Predominant strains of polycyclic aromatic hydrocarbon-degrading consortia from deep sea of the Middle Atlantic Ridge. Acta Microbiol Sin 49:902–909

    CAS  Google Scholar 

  • Cui ZS, Lai QL, Dong CM, Shao ZZ (2008) Biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the Middle Atlantic Ridge. Environ Microbiol 10:2138–2149

    Article  PubMed  CAS  Google Scholar 

  • Dong XZ, Cai MY (2001) Determinative manual for routine bacteriology. Scientific, Beijing

    Google Scholar 

  • Dyksterhouse SE, Gray JP, Herwig RP, Lara JC, Staley JT (1995) Cycloclasticus pugetii gen. nov., sp. nov., an aromatic hydrocarbon-degrading bacterium from marine sediments. Int J Syst Bacteriol 45:116–123

    Article  PubMed  CAS  Google Scholar 

  • Euzéby, J. P. (2012). List of bacterial names with standing in nomenclature. http://www.bacterio.cict.fr/. Accessed 20 June 2012

  • Gauthier MJ, Lafay B, Christen R, Fernandez L, Acquaviva M, Bonin P, Bertrand JC (1992) Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int J Syst Bacteriol 42:568–576

    Article  PubMed  CAS  Google Scholar 

  • Green DH, Bowman JP, Smith EA, Gutierrez T, Bolch CJS (2006) Marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. Int J Syst Evol Microbiol 56:523–527

    Article  PubMed  CAS  Google Scholar 

  • Guo B, Gu J, Ye YG, Tang YQ, Kida K, Wu XL (2007) Marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the South China Sea. Int J Syst Evol Microbiol 57:1970–1974

    Article  PubMed  CAS  Google Scholar 

  • Hedlund BP, Geiselbrecht AD, Staley JT (2001) Marinobacter santoriniensis sp. nov., an arsenate-respiring and arsenite-oxidizing bacterium isolated from hydrothermal sediment. Int J Syst Evol Microbiol 59:886–892

    Google Scholar 

  • Huu NB, Denner EBM, Ha DTC, Wanner G, Stan-Lotter H (1999) Marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a Vietnamese oil-producing well. Int J Syst Bacteriol 49:367–375

    Article  PubMed  CAS  Google Scholar 

  • Kim BY, Weon HY, Yoo SH, Kim JS, Kwon SW, Stackebrandt E, Go SJ (2006) Marinobacter koreensis sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 56:2653–2656

    Article  PubMed  CAS  Google Scholar 

  • Kiyohara H, Nagao K, Yana K (1982) Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Appl Environ Microbiol 43:454–457

    PubMed  CAS  Google Scholar 

  • Márquez MC, Ventosa A (2005) Marinobacter hydrocarbonoclasticus Gauthier et al. 1992 and Marinobacter aquaeolei Nguyen et al. 1999 are heterotypic synonyms. Int J Syst Evol Microbiol 55:1349–1351

  • Melcher RJ, Apitz SE, Hemmingsen BB (2002) Impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies. Appl Environ Microbiol 68:2858–2868

    Article  PubMed  CAS  Google Scholar 

  • Mesbah M, Whitman WB (1989) Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479:297–306

    Article  PubMed  CAS  Google Scholar 

  • Prakash O, Kumari K, Lal R (2007) Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. Int J Syst Evol Microbiol 57:527–531

    Article  PubMed  CAS  Google Scholar 

  • Qu LY, Zhu FL, Zhang JX, Gao CL, Sun XQ (2011) Marinobacter daqiaonensis sp. nov., a moderate halophile isolated from a Yellow Sea salt pond. Int J Syst Evol Microbiol 61:3003–3008

    Article  PubMed  CAS  Google Scholar 

  • Romanenko LA, Schumann P, Rohde M, Zhukova NV, Mikhailov VV, Stackebrandt E (2005) Marinobacter bryozoorum sp. nov. and Marinobacter sediminum sp. nov., novel bacteria from the marine environment. Int J Syst Evol Microbiol 55:143–148

    Article  PubMed  CAS  Google Scholar 

  • Rontani JF, Gilewicz MJ, Michotey VD, Zheng TL, Bonin PC, Bertrand JC (1997) Aerobic and anaerobic metabolism of 6,10,14-trimethylpentadecan-2-one by a denitrifying bacterium isolated from marine sediments. Appl Environ Microbiol 63:636–643

    PubMed  CAS  Google Scholar 

  • Rzhetsky A, Nei M (1992) A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9:945–967

    CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI Inc, Newark

    Google Scholar 

  • Shieh WY, Jean WD, Lin YT, Tseng M (2003) Marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in Lutao, Taiwan. Can J Microbiol 49:244–252

    Article  PubMed  CAS  Google Scholar 

  • Shivaji S, Gupta P, Chaturvedi P, Suresh K, Delille D (2005) Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 55:1453–1456

    Article  PubMed  CAS  Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Tourova TP, Antonov AS (1987) Identification of microorganisms by rapid DNA–DNA hybridization. Methods Microbiol 19:333–355

    Article  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Truper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

Download references

Acknowledgments

This work was financially supported by National Natural Science Foundation of China (40906062, 41076108), the Project of Fundamental Science Research, First Institute of Oceanography (2012T07, 2010G23), and Open Fund of Key Lab of Coastal Ecosystem and Environment Research of State Oceanic Administration (201008).

Author information

Authors and Affiliations

  1. Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration of China, Qingdao, 266061, People’s Republic of China

    Wei Gao, Zhisong Cui, Qian Li, Guangsu Xu, Xingjun Jia & Li Zheng

Authors
  1. Wei Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhisong Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qian Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guangsu Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xingjun Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Li Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Li Zheng.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 406 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gao, W., Cui, Z., Li, Q. et al. Marinobacter nanhaiticus sp. nov., polycyclic aromatic hydrocarbon-degrading bacterium isolated from the sediment of the South China Sea. Antonie van Leeuwenhoek 103, 485–491 (2013). https://doi.org/10.1007/s10482-012-9830-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-012-9830-z

Keywords

Search

Navigation