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Nocardioides panzhihuaensis sp. nov., a novel endophytic actinomycete isolated from medicinal plant Jatropha curcas L.

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Abstract

A novel Gram-positive, aerobic, rod-shaped and mycelia-producing bacterial strain, designated KLBMP 1050T, was isolated from the stem of the oil-seed plant Jatropha curcas L. collected from Sichuan Province, south-west China. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate KLBMP 1050T belonged to the genus Nocardioides, with the highest sequence similarity to Nocardioides albus KCTC 9186T (99.38 %) and Nocardioides luteus KCTC 9575T (99.03 %). However, the DNA–DNA relatedness of isolate KLBMP 1050T to these two type strains were 37.5 ± 3.5 and 33 ± 2.3 %, respectively. Strain KLBMP 1050T grew at the pH range 6–11, temperature range 10–32 °C and with 0–12 % NaCl. The physiological properties of strain KLBMP 1050T differ from those of N. albus KCTC 9186T and N. luteus KCTC 9575T. The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-8(H4) was the major respiratory quinone. The predominant cellular fatty acid of strain KLBMP 1050T was iso-C16:0 (23.3 %). The total DNA G+C content was 70.1 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain KLBMP 1050T represents a novel species of the genus Nocardioides, for which the name Nocardioides panzhihuaensis sp. nov. is proposed. The type strain is KLBMP 1050T (= KCTC 19888T = NBRC 108680T).

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Acknowledgments

The authors are grateful to KCTC for kindly providing the type strain N. albus KCTC 9186T and Prof. Iain C. Sutcliffe for his valuable comments on the manuscript. This research was partially supported by National Natural Science Foundation of China (31000005, 31101502), the Program of Natural Science Foundation of the Jiangsu Higher Education Institutions of China (11KJD210002), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), the Project of Outstanding Scientific and Technological Innovation Team for Higher Education Institutions in Jiangsu Province (Pre-development of medical microbiology) and Grants from Natural Science Foundation by Xuzhou City (XZZD1004).

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Authors and Affiliations

  1. The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, People’s Republic of China

    Sheng Qin, Bo Yuan, Yue-Ji Zhang, Guang-Kai Bian, Bing-Zhi Sun & Ji-Hong Jiang

  2. The Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Wen-Jun Li

  3. NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan

    Tomohiko Tamura

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  1. Sheng Qin
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  2. Bo Yuan
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Correspondence to Wen-Jun Li or Ji-Hong Jiang.

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Qin, S., Yuan, B., Zhang, YJ. et al. Nocardioides panzhihuaensis sp. nov., a novel endophytic actinomycete isolated from medicinal plant Jatropha curcas L.. Antonie van Leeuwenhoek 102, 353–360 (2012). https://doi.org/10.1007/s10482-012-9745-8

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