Abstract
Lipomyces starkeyi is an oleaginous yeast, and has been classified in four distinct groups, i.e., sensu stricto and custers α, β, and γ. Recently, L. starkeyi clusters α, β, and γ were recognized independent species, Lipomyces mesembrius, Lipomyces doorenjongii, and Lipomyces kockii, respectively. In this study, we investigated phylogenetic relationships within L. starkeyi, including 18 Japanese wild strains, and its related species, based on internal transcribed spacer sequences and evaluated biochemical characters which reflected the phylogenetic tree. Phylogenetic analysis showed that most of Japanese wild strains formed one clade and this clade is more closely related to L. starkeyi s.s. clade including one Japanese wild strain than other clades. Only three Japanese wild strains were genetically distinct from L. starkeyi. Lipomyces mesembrius and L. doorenjongii shared one clade, while L. kockii was genetically distinct from the other three species. Strains in L. starkeyi s.s. clade converted six sugars, d-glucose, d-xylose, l-arabinose, d-galactose, d-mannose, and d-cellobiose to produce high total lipid yields. The Japanese wild strains in subclades B, C, and D converted d-glucose, d-galactose, and d-mannose to produce high total lipid yields. Lipomyces mesembrius was divided into two subclades. Lipomyces mesembrius CBS 7737 converted d-xylose, l-arabinose, d-galactose, and d-cellobiose, while the other L. mesembrius strains did not. Lipomyces doorenjongii converted all the sugars except d-cellobiose. In comparison to L. starkeyi, L. mesembrius, and L. doorenjongii, L. kockii produced higher total lipid yields from d-glucose, d-galactose, and d-mannose. The type of sugar converted depended on the subclade classification elucidated in this study.
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Acknowledgments
We thank Mr. Naoto Wakamura for his technical assistance. This study was supported by the New Energy and Technology Development Organization (NEDO), Japan.
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10482_2011_9641_MOESM1_ESM.ppt
Fig. S1 in supplementary material Scatter diagram of first two components from a principal component analysis with 12 biochemical characters. L. starkeyi s.s. clades = open circle, wild strains in subclade B = open square, wilds strains in subclade C = solid squere, wild strains in subclade D = solid circle, L. mesembrius CBS 7661T, CBS 7600, and CBS 7601 = open diamond, L. mesembrius CBS 7737 = solid diamond, L. doorenjongii = open triangle, L. kockii = cross. (PPT 266 kb)
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Oguri, E., Masaki, K., Naganuma, T. et al. Phylogenetic and biochemical characterization of the oil-producing yeast Lipomyces starkeyi . Antonie van Leeuwenhoek 101, 359–368 (2012). https://doi.org/10.1007/s10482-011-9641-7
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DOI: https://doi.org/10.1007/s10482-011-9641-7