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tdd8: a TerD domain-encoding gene involved in Streptomyces coelicolor differentiation

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Abstract

The Streptomyces coelicolor genome contains 17 TerD domain-encoding genes (tdd genes) of unknown function. The proteins encoded by these genes have been presumed to be involved in tellurite resistance on the basis of their homology with the protein TerD of Serratia marcescens. To elucidate the role of a Tdd protein (Tdd8), both a deletion mutant for the corresponding gene tdd8 (SCO2368) and a recombinant strain over-expressing tdd8 were produced in S. coelicolor M145. The deletion mutant (Δtdd8), like the wild strain, was not resistant to potassium tellurite. The deletion was not lethal but had a marked effect on differentiation. The deletion strain showed more rapid growth in liquid medium and produced long chains of short spores with a dense and non-spherical spore wall on agar plates. The strain over-expressing tdd8 had a growth delay in liquid medium and produced very few spores of irregular shapes and sizes on solid medium. The results of this study demonstrated that Tdd proteins might have a function other than tellurite resistance and this function seems to be of crucial importance for the proper development of the actinomycete S. coelicolor.

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Acknowledgments

This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to CB. ÉS gratefully acknowledges the receipt of scholarships from NSERC and the Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT). The authors thank Yinhua Lu for the gift of plasmid pSET152 m, Karine Robert, Isabelle Madore, Irène Kelsey and Anne-Marie Simao-Beaunoir for technical assistance and Ryszard Brzezinski for critical reading of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Centre d’Étude et de Valorisation de la Diversité Microbienne, Département de biologie, Université de Sherbrooke, Sherbrooke, QC, J1K 2R1, Canada

    Édith Sanssouci, Sylvain Lerat, Gilles Grondin & Carole Beaulieu

  2. INRS-Institut Armand Frappier, Laval, QC, H7V 1B7, Canada

    François Shareck

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  1. Édith Sanssouci
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  2. Sylvain Lerat
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  3. Gilles Grondin
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  5. Carole Beaulieu
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Correspondence to Carole Beaulieu.

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Sanssouci, É., Lerat, S., Grondin, G. et al. tdd8: a TerD domain-encoding gene involved in Streptomyces coelicolor differentiation. Antonie van Leeuwenhoek 100, 385–398 (2011). https://doi.org/10.1007/s10482-011-9593-y

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