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Porifericola rhodea gen. nov., sp. nov., a new member of the phylum Bacteroidetes isolated by the bait-streaked agar technique

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Abstract

A strictly aerobic, gram-negative, non-motile, reddish-pink pigmented, rod-shaped strain designated N5EA6-3A2BT, was isolated from an unidentified marine sponge by use of a bait-streaked agar technique. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolate represented a distinct and deep evolutionary lineage of descent in the family Flammeovirgaceae within the phylum Bacteroidetes and clustered with as yet uncultured bacteria. The most closely related established species was Roseivirga spongicola UST030701-084T (89% sequence similarity) in the family of Flammeovirgaceae. The strain could be differentiated phenotypically and physiologically from recognized members of the family Flammeovirgaceae. The G+C content of DNA was 43 mol%, the major respiratory quinone was menaquinone 7 (MK-7) and iso-C15:0, C16:1ω5c and iso-C17:0 3-OH were the major fatty acids. From the distinct phylogenetic position and combination of genotypic and phenotypic characteristics, the name Porifericola rhodea gen. nov., sp. nov. is proposed. The type strain of Porifericola rhodea is N5EA6-3A2BT (=MBIC08357T = NBRC 107748T).

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Acknowledgments

The authors thank Atsuko Katsuta for technical assistance. This study was supported by the New Energy and Industrial Technology Development Organization (NEDO), and in part by a research grant (2009–2011) of the Institute for Fermentation, Osaka, Japan. The present research has also been conducted by the Bisa Research Grant of Keimyung University in 2011.

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Author notes

  1. Naoya Oku

    Present address: Biotechnology Research Center, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan

  2. Hiroaki Kasai

    Present address: Marine Biosciences Kamaishi Research Laboratory, Kitasato University, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

Authors and Affiliations

  1. Department of Pharmacy, College of Pharmacy, Keimyung University, 2800 Dalgubeoldaero, Dalseo-Gu, Daegu, 704-701, Republic of Korea

    Jaewoo Yoon

  2. Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan

    Jaewoo Yoon

  3. Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-0032, Japan

    Sanghwa Park & Akira Yokota

  4. Marine Biotechnology Institute Co. Ltd, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan

    Naoya Oku & Hiroaki Kasai

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  1. Jaewoo Yoon
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Correspondence to Jaewoo Yoon.

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Yoon, J., Oku, N., Park, S. et al. Porifericola rhodea gen. nov., sp. nov., a new member of the phylum Bacteroidetes isolated by the bait-streaked agar technique. Antonie van Leeuwenhoek 100, 145–153 (2011). https://doi.org/10.1007/s10482-011-9575-0

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