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Pseudonocardia rhizophila sp. nov., a novel actinomycete isolated from a rhizosphere soil

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Abstract

During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 67013T was isolated from rhizosphere soil of Tripterygium wilfordii Hook. f. collected from Yunnan province, south-west China. The strain formed well-differentiated aerial and substrate mycelia, grew in the presence of up to 15% NaCl (optimum, 0–3% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 67013T belongs to the genus Pseudonocardia, with highest similarity to Pseudonocardia thermophila DSM 43832T (97.6%). Sequence similarities between strain YIM 67013T and the other Pseudonocardia species ranged from 96.6 to 93.2%. Key morphological and physiological characteristics as well as chemotaxonomic features of strain YIM 67013T were congruent with the description of the genus Pseudonocardia. The G+C content of the genomic DNA was 69.7 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including low DNA–DNA hybridization results, it is proposed that strain YIM 67013T represents a novel species of the genus Pseudonocardia, named Pseudonocardia rhizophila sp. nov. The type strain is YIM 67013T (= CCTCC AA 209043T = DSM 45381T).

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Acknowledgements

This research was supported by the National Basic Research Program of China (No. 2010CB833800), National Natural Science Foundation of China (No. U0932601), Key Project of International Cooperation (2007DFB31620) and Yunnan Provincial Natural Science Foundation (Nos. 2009AC017 and 2009DA002).

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Authors and Affiliations

  1. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Jie Li, Guo-Zhen Zhao, Hai-Yu Huang, Wen-Yong Zhu, Li-Hua Xu & Wen-Jun Li

  2. Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Jie Li, Guo-Zhen Zhao, Hai-Yu Huang, Wen-Yong Zhu, Li-Hua Xu & Wen-Jun Li

  3. Korea Research Institutes of Biosciences and Biotechnology, 52, Eoeun-dong, Yuseong gu, Daejeon, 305-333, Korea

    Jae-Chan Lee & Chang-Jin Kim

  4. Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Li-Xin Zhang & Wen-Jun Li

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  1. Jie Li
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Correspondence to Wen-Jun Li.

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Li, J., Zhao, GZ., Huang, HY. et al. Pseudonocardia rhizophila sp. nov., a novel actinomycete isolated from a rhizosphere soil. Antonie van Leeuwenhoek 98, 77–83 (2010). https://doi.org/10.1007/s10482-010-9431-7

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