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Usefulness of Multiple-Locus VNTR Fingerprinting in detection of clonality of community- and hospital-acquired Staphylococcus aureus isolates

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Abstract

Staphylococcus aureus has become a major source of hospital infections and the risk of colonisation and infection by community-acquired methicillin-resistant S. aureus (CA-MRSA) is increasingly higher. Because of the importance of S. aureus to public health, many molecular typing methods have been developed to determine its transmission routes and source of infection during epidemiological investigations. In this study we evaluated the usefulness of multiplex PCR based Multi-Locus VNTR Fingerprinting (MLVF) as the first step method for rapid differentiation of Croatian and Polish S. aureus isolates in hospital and community settings. This is a first report of the usefulness of MLVF in typing of hospital-acquired methicillin-sensitive S. aureus (HA-MSSA) and four CA-MRSA isolates. A total of 47 isolates of S. aureus recovered in Croatia in 2004 and in Poland in 2006 and 2007 were tested. The MLVF results were compared to those produced by other typing methods, such as Pulsed-Field Gel Electrophoresis (PFGE), Multi-Locus Sequence Typing (MLST) and spa typing. The MLVF analysis showed almost the same clonality results as the remaining typing methods although some differences were found. Epidemiological data about the relation among S. aureus isolates and the results produced by typing methods applied in the present study indicate that because of the advantages in ease and speed of Variable Number of Tandem Repeats (VNTR) procedure over PFGE, spa typing and MLST, MLVF can be used as a first screening method followed by additional typing.

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Acknowledgements

These studies were supported by grant 2 P05A 052 30 (to A. S. and W. H.). We are grateful to Herminia de Lencastre and Alexander Tomasz for kindly providing representatives of the MRSA international clones. We would like to thank Aleksandra Kozinska and Katarzyna Malinowska for excellent technical assistance. We acknowledge the use of the S. aureus MLST database which is located at Imperial College, London, and is founded by the WellcomeTrust. This publication made use of the spa typing website (http://www.spaserver.ridom.de/) and Ridom Staph Type software that is developed by Ridom GmbH and curated by SeqNet.org (http://www.SeqNet.org/). This study was presented in part as a poster at the Twelfth International Symposium on Staphylococci and Staphylococcal Infections, Maastricht, the Netherlands 3–6 September 2006.

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  1. Artur Sabat

    Present address: University Medical Center Groningen, University of Groningen, Groningen, The Netherlands

Authors and Affiliations

  1. Centre of Quality Control in Microbiology, Chełmska 30/34, 00-725, Warsaw, Poland

    Agnieszka Luczak-Kadlubowska & Waleria Hryniewicz

  2. National Medicines Institute, Warsaw, Poland

    Artur Sabat, Jolanta Krzyszton-Russjan & Waleria Hryniewicz

  3. The University Hospital for Infectious Diseases, Zagreb, Croatia

    Arjana Tambic-Andrasevic

  4. Public Health Laboratory, Cakovec, Croatia

    Marina Payerl-Pal

Authors
  1. Agnieszka Luczak-Kadlubowska
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Correspondence to Agnieszka Luczak-Kadlubowska.

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Luczak-Kadlubowska, A., Sabat, A., Tambic-Andrasevic, A. et al. Usefulness of Multiple-Locus VNTR Fingerprinting in detection of clonality of community- and hospital-acquired Staphylococcus aureus isolates. Antonie van Leeuwenhoek 94, 543–553 (2008). https://doi.org/10.1007/s10482-008-9271-x

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