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Wolinella succinogenes response to ox-bile stress

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Abstract

The bacterium Wolinella succinogenes is the only known species of its genus. It was first isolated from cow ruminal fluid, and in cattle, it dwells in the reticulum and rumen compartments of the stomach. The global protein response of W. succinogenes to ox-bile was investigated with the aim to understand bile-tolerance mechanisms of the bacterium. Bacteria were grown in liquid media supplemented with different bile concentrations to determine its effects on growth and morphology. Proteomic analyses served to identify 14 proteins whose expression was modulated by the presence of 0.2% bile. Quantitative real-time PCR analyses of the expression of selected genes were employed to obtain independent confirmation of the proteomics data. Proteins differentially expressed revealed metabolic pathways involved in the adaptation of W. succinogenes to bile. The data suggested that bile stress elicited complex physiological responses rather than just specific pathways, and identified proteins previously unknown to be involved in the adaptation of bacteria to bile.

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Acknowledgements

The authors are grateful to Mr Edward Fox, Mr Arinze Okoli and Mr Nadeem Kaakoush for valuable discussions and suggestions.

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Authors and Affiliations

  1. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW, 2052, Australia

    Bernice M. Tan

  2. School of Medical Sciences, The University of New South Wales, Sydney, NSW, 2052, Australia

    Quoc V. Tu, Zsuzsanna Kovach & George L. Mendz

  3. Bioanalytical Mass Spectrometry Facility, The University of New South Wales, Sydney, NSW, 2052, Australia

    Mark Raftery

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  1. Bernice M. Tan
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  2. Quoc V. Tu
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  3. Zsuzsanna Kovach
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  4. Mark Raftery
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  5. George L. Mendz
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Correspondence to George L. Mendz.

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Tan, B.M., Tu, Q.V., Kovach, Z. et al. Wolinella succinogenes response to ox-bile stress. Antonie van Leeuwenhoek 92, 319–330 (2007). https://doi.org/10.1007/s10482-007-9151-9

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  • DOI: https://doi.org/10.1007/s10482-007-9151-9

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