Skip to main content
SpringerLink
Log in

The sequence of the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway in Penicillium species

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

Interest in species of the genus Penicillium is related to their ability to produce the mycotoxin patulin and to cause spoilage of fruit products worldwide. The sequence of the isoepoxydon dehydrogenase (idh) gene, a gene in the patulin biosynthetic pathway, was determined for 28 strains representing 12 different Penicillium species known to produce the mycotoxin patulin. Isolates of Penicillium carneum, Penicillium clavigerum, Penicillium concentricum, Penicillium coprobium, Penicillium dipodomyicola, Penicillium expansum, Penicillium gladioli, Penicillium glandicola, Penicillium griseofulvum, Penicillium paneum, Penicillium sclerotigenum and Penicillium vulpinum were compared. Primer pairs for DNA amplification and sequencing were designed from the P. griseofulvum idh gene (GenBank AF006680). The two introns present were removed from the nucleotide sequences, which were translated to produce the IDH sequences of the 12 species for comparison. Phylogenetic relationships among the species were determined from rDNA (ITS1, 5.8 S, ITS2 and partial sequence of 28S rDNA) and from the idh nucleotide sequences minus the two introns. Maximum parsimony analysis showed trees based on rDNA and idh sequences to be congruent. It is anticipated that the genetic information obtained in the present study will aid in the design of probes, specific for patulin biosynthetic pathway genes, to identify the presence of these mycotoxigenic fungi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Beck J, Ripka S, Siegner A, Schiltz E, Schweizer E (1990) The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum. Its gene structure relative to that of other polyketide synthases. Eur J Biochem 192:487–498

    Article  PubMed  CAS  Google Scholar 

  • Boysen M, Skouboe P, Frisvad JC, Rossen L (1996) Reclassification of the Penicillium roqueforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiology 142:541–549

    Article  PubMed  CAS  Google Scholar 

  • Dombrink-Kurtzman MA (2006) The isoepoxydon dehydrogenase gene of the patulin metabolic pathway differs for Penicillium griseofulvum and Penicillium expansum. Anton van Leeuw 89:1–8

    Article  CAS  Google Scholar 

  • Dombrink-Kurtzman MA, Blackburn JA (2005) Evaluation of several cultural media for production of patulin by Penicillium species. Int J Food Microbiol 98:241–248

    Article  PubMed  CAS  Google Scholar 

  • Dombrink-Kurtzman MA, Engberg AE (2006) Byssochlamys nivea with patulin producing capability has an isoepoxydon dehydrogenase gene (idh) with sequence homology to Penicillium expansum and P. griseofulvum. Mycol Res 110:1111–1118

    Article  CAS  Google Scholar 

  • Fedeshko RW (1992) Polyketide enzymes and genes in Penicillium urticae. Ph.D. Dissertation, University of Calgary, Calgary, Alberta, Canada

  • Frisvad JC, Filtenborg O (1983) Classification of terverticillate penicillia based on profiles of mycotoxins and other secondary metabolites. Appl Environ Microbiol 46:1301–1310

    PubMed  CAS  Google Scholar 

  • Frisvad JC, Filtenborg O (1989) Terverticillate penicillia: chemotaxonomy and mycotoxin production. Mycologia 81:837–861

    CAS  Google Scholar 

  • Frisvad JC, Samson RA (2004) Polyphasic taxonomy of Penicillium subspecies Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Stud Mycol 49:1–174

    Google Scholar 

  • Frisvad JC, Thrane U (1987) Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV–VIS spectra (diode array detection). J Chromatogr 404:195–214

    Article  PubMed  CAS  Google Scholar 

  • Frisvad JC, Filtenborg O, Wicklow DT (1987) Terverticillate Penicillia isolated from underground seed caches and cheek pouches of banner-tailed kangaroo rats (Dipodomys spectabilis). Can J Bot 65:765–773

    CAS  Google Scholar 

  • Frisvad JC, Smedsgaard J, Larsen TO, Samson RA (2004) Mycotoxins, drugs and other extrolites produced by species in Penicillium subgenus Penicillium. Stud Mycol 49:201–242

    Article  Google Scholar 

  • Leistner L, Eckardt C (1979) Vorkommen toxinogener Penicillien bei Fleischerzeugnissen. Fleischwirtschaft 59:1892–1896

    Google Scholar 

  • Paterson RRM, Archer S, Kozakiewicz Z, Lea A, Locke T, O’Grady E (2000) A gene probe for the patulin metabolic pathway with potential for use in patulin and novel disease control. Biocontrol Sci Technol 10:509–512

    Article  Google Scholar 

  • Paterson RRM, Kozakiewicz Z, Locke T, Brayford D, Jones SCB (2003) Novel use of the isoepoxydon dehydrogenase gene probe of the patulin metabolic pathway and chromatography to test penicillia isolated from apple production systems for the potential to contaminate apple juice with patulin. Food Microbiol 20:359–364

    Article  CAS  Google Scholar 

  • Peterson SW (2000) Phylogenetic analysis of Penicillium species based on ITS and LSU-rDNA nucleotide sequences. In: Samson RA, Pitt JI (eds) Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Harwood Academic Publishers, Amsterdam, pp 163–178

    Google Scholar 

  • Scrutton NS, Berry A, Perham RN (1990) Redesign of the coenzyme specificity of a dehydrogenase by protein engineering. Nature 343:38–43

    Article  PubMed  CAS  Google Scholar 

  • Sekiguchi J, Gaucher GM (1978) Identification of phyllostine as an intermediate of the patulin pathway in Penicillium urticae. Biochemistry 19:1785–1791

    Article  Google Scholar 

  • Sekiguchi J, Gaucher GM (1979) Isoepoxydon, a new metabolite of the patulin pathway in Penicillium urticae. Biochem J 182:445–453

    PubMed  CAS  Google Scholar 

  • Sekiguchi J, Shimamoto T, Yamada Y, Gaucher GM (1983) Patulin biosynthesis: enzymatic and nonenzymatic transformations of the mycotoxin (E)-ascladiol. Appl Environ Microbiol 45:1939–1942

    PubMed  CAS  Google Scholar 

  • Svendsen A, Frisvad JC (1994) A chemotaxonomic study of the terverticillate Penicillia based on high performance liquid chromatography of secondary metabolites. Mycol Res 98:1317–1328

    Article  CAS  Google Scholar 

  • Swofford DL (1998) PAUP* 4.0: phylogenetic analysis using parsimony (and other methods). Sinauer Associates, Sunderland, MA, USA

    Google Scholar 

  • White S, O’ Callaghan J, Dobson ADW (2006) Cloning and molecular characterization of Penicillium expansum genes upregulated under conditions permissive for patulin biosynthesis. FEMS Microbiol Lett 255:17–26

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

Special thanks are given to Amy E. Engberg for excellent technical assistance and to Dr. Cletus P. Kurtzman for generation of phylogenetic trees. Names of equipment and chemical supplies are necessary to report factually on experimental methods; however, the USDA neither guarantees nor warrants the standard of the products, and the use of the name by USDA implies no approval of the products to the exclusion of others that may also be suitable.

Author information

Authors and Affiliations

  1. Mycotoxin Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL, 61604-3902, USA

    Mary Ann Dombrink-Kurtzman

Authors
  1. Mary Ann Dombrink-Kurtzman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mary Ann Dombrink-Kurtzman.

Additional information

The U.S. Government's right to retain a non-exclusive, royalty-free license in and to any copyright is acknowledged.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dombrink-Kurtzman, M.A. The sequence of the isoepoxydon dehydrogenase gene of the patulin biosynthetic pathway in Penicillium species. Antonie van Leeuwenhoek 91, 179–189 (2007). https://doi.org/10.1007/s10482-006-9109-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-006-9109-3

Keywords

Search

Navigation