Abstract
The first description of the species Aeromonas allosaccharophila was only based on two strains (the type strain CECT4199, and a duplicate CECT4200) isolated from diseased elvers (Anguilla anguilla) of an eel-farm located in Valencia, Spain, and one stool isolate (ATCC35942) from a female with diarrhoea and food poisoning in South Carolina, U.S.A. In the present study, 17 Aeromonas isolates obtained from carcasses of pigs and from the equipment for the cleaning process, and one strain recently isolated from a clinical case of gastroenteritis, were genetically identified as Aeromonas allosaccharophila on the basis of gyrB and 16S rRNA gene sequencing. In addition, this phylogenetic approach also supports the classification of Aeromonas veronii biogroup sobria reference strains LMG13071, LMG13073 and LMG13074 within the species A. allosaccharophila. The A. allosaccharophila strains isolated from pig carcasses processed in a single slaughterhouse presented a clonal origin, on the basis of random amplified polymorphic DNA genetic typing. To our knowledge, this is the first time since the species description that A. allosaccharophila has been newly identified, being on this occasion isolated from the environment of a slaughterhouse. Our findings indicate that this species may be readily identified by a sequencing approach and, consequently, the present work supports the existence of this phylogenetic cluster.
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References
Altwegg M (1999) Aeromonas and Plesiomonas. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds) Manual of clinical microbiology. American Society for Microbiology, Washington, D.C., pp 507–516
Austin B, Adams C (1996) Fish pathogens. In: Austin B, Altwegg M, Gosling PJ, Joseph S (eds) The genus Aeromonas. John Wiley & Sons Ltd., Chichester, England, pp 197–244
Clayton RA, Sutton G, Hinkle PS Jr, Bult C, Fields C (1995) Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa. Int J Syst Bacteriol 45:513–523
Esteve C, Gutiérrez MC, Ventosa A (1995) DNA relatedness among Aeromonas allosaccharophila strains and DNA hybridization groups of the genus Aeromonas. Int J Syst Bacteriol 45:390–391
Esteve C, Valera L, Gutiérrez C, Ventosa A (2003) Taxonomic study of sucrose-positive Aeromonas jandaei-like isolates from faeces, water and eels: emendation of A. jandaei Carnahan et al. 1992. Int J Syst Evol Microbiol 53:1411–1419
Figueras MJ, Suarez-Franquet A, Chacón MR, Soler L, Navarro M, Alejandre C, Grasa B, Martínez-Murcia AJ, Guarro J (2005) First record of the rare species Aeromonas culicicola from a drinking water supply. Appl Environ Microbiol 65:5293–5302
Gill CO, JonesT (1995) Occurrence of Aeromonas, Listeria and Yersinia in carcass processing equipment at two slaughtering plants. Food Microbiol 12:135–141
Gurtler V, Mayall BC (2001) Genomic approaches to typing, taxonomy and evolution of bacterial isolates. Int J Syst Evol Microbiol 51:3–16
Hickman-Brenner FW, MacDonald KL, Steigerwalt AG, Fanning GR, Brenner DJ, Farmer JJ III (1987) Aeromonas veronii, a new ornithine decarboxylase-positive species that may cause diarrhoea. J Clin Microbiol 25:900–906
Huys G, Coopman R, Janssen P, Kersters K (1996) High-resolution genotypic analysis of the genus Aeromonas by AFLP fingerprinting. Int J Syst Bacteriol 46:572–580
Huys G, Kämpfer P, Swing J (2001) New DNA-DNA hybridization and phenotypic data on the species Aeromonas ichthiosmia and Aeromonas allosaccharophila: A. ichthiosmia Shubert et al. 1990 is a later synonym of A. veronii Hickman-Brenner et al. 1987. Syst Appl Microbiol 24:177–182
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Kirov SM (1993) The public health significance of Aeromonas spp. in foods. Int J Food Microbiol 20:179–198
Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 50:602–612
Martínez-Murcia AJ (1999) Phylogenetic positions of Aeromonas encheleia, Aeromonas popoffii, Aeromonas DNA hybridization Group 11 and Aeromonas Group 501. Int J Syst Bacteriol 49:1403–1408
Martínez-Murcia AJ, Benlloch S, Collins MD (1992a) Phylogenetic interrelationships of members of the genera Aeromonas and Plesiomonas as determined by 16S ribosomal DNA sequencing: lack of congruence with results of DNA-DNA hybridizations. Int J Syst Microbiol 42:412–421
Martínez-Murcia AJ, Esteve C, Garay E, Collins MD (1992b) A. Allosaccharophila sp. nov., a new mesophilic member of the genus Aeromonas. FEMS Microbiol Lett 91:199–206
Martínez-Murcia AJ, Boán IF, Rodríguez-Valera F (1995) Evaluation of the authenticity of haloarchaeal strains by random-amplified polymorphic DNA. Lett Appl Microbiol 21:106–108
Martínez-Murcia AJ, Antón AI, Rodriguez-Valera F (1999) Patterns of sequence variation in two regions of the 16S rRNA multigene family of Escherichia coli. Int J Syst Bacteriol 49:601–610
Martínez-Murcia AJ, Soler L, Saavedra MJ, Chacón MR, Guarro J, Stackebrandt E, Figueras MJ (2005) Phenotypic, genotypic and phylogenetic discrepancies to differentiate Aeromonas salmonicida and Aeromonas bestiarum from each other. Int Microbiol 8:259–269
Morandi A, Zhaxybayeva O, Gogarten JP, Graf J (2005) Evolutionary and diagnostic implications of intragenomic heterogeneity in the 16S rRNA gene in Aeromonas strains. J Bacteriol 187:6561–6564
Saavedra MJ, Guedes-Novais S, Alves A, Rema P, Tacão M, Correia A, Martínez-Murcia AJ (2004) Resistance to β-lactam antibiotics in Aeromonas hydrophila isolated from rainbow trout (Oncorhynchus mykiss). Int Microbiol 7:201–211
Saitou N, Nei M (1987) The Neighbor-Joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Soler L, Yañez A, Chacón MR, Aguilera-Arreola MG, Catalán V, Figueras MJ, Martínez-Murcia AJ (2004) Phylogenetic analysis of the genus Aeromonas based on two housekeeping genes. Int J Syst Evol Microbiol 54:1511–1519
Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfer P, Maiden MCJ, Nesme X, Roselló-Mora R, Swings J, Trüper HG, Vauterin L, Ward AC, Whitman WB (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higging DH (1997) The CLUSTAL X window interface: flexible strategies for multiple sequences alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Yáñez MA, Catalán V, Apráiz D, Figueras MJ, Martínez-Murcia AJ (2003) Phylogenetic analysis of members of the genus Aeromonas based on GyrB gene sequences. Int J Syst Evol Microbiol 53:875–883
Acknowledgements
This work has been supported by grants IMIDTA/2004/369 from IMPIVA, Generalitat Valenciana. M.J. Saavedra was recipient of a grant (SFRH/BSAB/439/2004) from the Fundação para a Ciência e Tecnologia. We are most grateful to Drs Navarro and Gonzalo for the strain MDC561.
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Saavedra, M.J., Perea, V., Fontes, M.C. et al. Phylogenetic identification of Aeromonas strains isolated from carcasses of pig as new members of the species Aeromonas allosaccharophila . Antonie van Leeuwenhoek 91, 159–167 (2007). https://doi.org/10.1007/s10482-006-9107-5
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DOI: https://doi.org/10.1007/s10482-006-9107-5