Abstract
Micromonospora echinospora differentiates in both submerged and surface cultures producing abundant dark spores after a period of vegetative mycelial growth. In submerged batch cultures, under either carbon or nitrogen limiting conditions, protease activity was found to coincide with sporulation indicating a relationship between proteolytic activity and differentiation in this organism. Further evidence for this link was provided from surface grown cultures wherein sporulation was inhibited by the serine protease inhibitors TLCK and TPCK. The association between proteolysis and differentiation apparent in this organism correlates with evidence of a similar phenomenon observed in the streptomycetes, suggesting that this may be a common response associated with differentiation in filamentous actinomycetes.
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References
Bradford M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254
Brock F.M., Forsberg C.W., Buchanan-Smith J.G. (1982). Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors. Appl. Env. Microbiol. 44: 561–569
Butler M.J., Aphale J.S., Binnie C., Di Zonno M.A., Krygsman P., Soltes G., Walczyk E., Malek L.T. (1996). Cloning and analysis of a gene from Streptomyces lividans 66 encoding a novel secreted protease exhibiting homology to subtilisn BPN. App. Micro. Biotech. 45: 141–147
Dunn B.M. 1989. Dertermination of protease mechanism. In: R.J. Beynon and Bond J.S. (eds.), Proteolytic Enzymes: A Practical Approach, Oxford University Press, Oxford
Fernandez M., Sanchez J. (2004) Nuclease activities and cell death processes associated with the development of surface cultures of Streptomyces antibioticus ETH 7451. Microbiology 148: 405–12
Gibb G.D., Strohl W.R. (1987). Physiological regulation of Protease activity in Streptomyces peucetius. Can. J. Microbiol. 34: 187–190
Ginter C.L. (1978). Sporulation and the production of serine protease and cephamicin C by Streptomyces lacamadurans. Antimicrob. Agents Chemother. 15: 522–526
Gottesman S. (1999). Regulation by proteolysis: Developmental switches. Curr. Op. Microbiol. 2: 142–147
Hoskisson P.A., Hobbs G., Sharples G.P. (2000). Sensitivity of Micromonospora echinospora (NCIMB 12744) spores to heat treatment with further evidence of a heat activation phenomenon. Lett. App. Micro. 30, 114–117
Hoskisson P.A. 2002. Growth, Development and Antibiotic production in Micromonospora echinospora (ATCC15837). Ph.D Thesis, Liverpool John Moores University
Hoskisson P.A., England R.R., Sharples G.P., Hobbs G. (2004). Modulation of glycogen and trehalose levels in Micromonospora echinospora (ATCC 15837). Antonie van Leeuwenhoek, 86, 225–233
Kang S.G., Kim I.S., Rho Y.T., Lee K.J. (1995). Production of extracellular proteases accompanying morphological differentiation of Streptomyces albidoflavus SMF301. Microbiology 141: 3095–3103
Kato J.Y., Chi W.J., Ohnishi Y., Hong S.K., Horinouchi S. (2005) Transcriptional control by A-factor of two trypsin genes in Streptomyces griseus. J Bacteriol. 187: 286–95
Kieser T., Bibb M.J., Buttner M.J., Chater K.F., Hopwood D.A. (2000).Practical Streptomyces genetics. John Innes foundation. Norwich
Kim I.S., Lee K.J. (1996). Trypsin-like protease of Streptomyces exfoliatus SMF13, a potential agent in mycelial differentiation. Microbiology 142: 1797–1806
Miguelez E.M., Hardisson C., Manzanal M.B. (1999). Hyphal death during colony development in Streptomyces antibioticus: morphological evidence for the existence of a process of cell deletion in a multicellular prokaryote. J Cell Biol. 145: 515–25
Nicieza RG, Huergo J, Connolly BA, Sanchez J (1999). Purification, characterization, and role of nucleases and serine proteases in Streptomyces differentiation. Analogies with the biochemical processes described in late steps of eukaryotic apoptosis. J Biol Chem. 274: 20366–75
Ochi K. (1985). Sporulation and antibiotic production by Bacillus subtilis mutants defective in intracellular proteases. Agric. Biol. Chem., 49, 905–907
Piggot P.J., Coote J.G. (1976). Genetic aspects of bacterial endospore formation. Bact. Rev. 40: 908–962
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Hoskisson, P.A., Sharples, G.P. & Hobbs, G. Differentiation and protease production in Micromonospora echinospora (ATCC 15837). Antonie Van Leeuwenhoek 89, 191–195 (2006). https://doi.org/10.1007/s10482-005-9001-6
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DOI: https://doi.org/10.1007/s10482-005-9001-6