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Sendai viral vector mediated angiopoietin-1 gene transfer for experimental ischemic limb disease

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Abstract

Sendai virus vector is emerging as a promising vector for gene therapy, and angiopoietin-1 (Ang-1) has been reported to improve the blood flow recovery in the ischemic limb or heart. In this study, we constructed a human Ang-1-expressing Sendai viral vector (SeVhAng-1) and injected it into the ischemic limb of rats. We found that SeVhAng-1 improved the blood flow recovery and increased the capillary density of the ischemic limb, compared with the controls. We also found that SeVhAng-1 increased p-Akt during the early period of limb ischemia, and decreased apoptosis in ischemic limb. It suggests that SeVhAng-1 may serve as a potential therapeutic tool in ischemic limb disease.

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Acknowledgments

This work was partly supported by a grant to HH from the Ministry of Education, Science, Japan. We thank Seiji Ohtani for his expert help in histological analysis, and Noriko Kawano for her help in performing the animal experiments. We also thank Takeo Yamamoto for his technical assistance in vector construction.

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Authors and Affiliations

  1. Department of Molecular Medicine, Sapporo Medical University, South 1, West 17, Chuo-ku, Sapporo, 060-8556, Japan

    Jianhua Huang, Kei Tomihara, Toshihiro Tanaka & Hirofumi Hamada

  2. Department of Thoracic and Cardiovascular Surgery, NingXia People’s Hospital, 148# HuaiYuan West Road, XiXia District, 750021, YingChuan, China

    Jianhua Huang & Jiahua Chen

  3. DNAVEC Research Corporation, Tsukuba, Ibaraki, Japan

    Makoto Inoue & Mamoru Hasegawa

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  1. Jianhua Huang
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  2. Makoto Inoue
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  3. Mamoru Hasegawa
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  7. Hirofumi Hamada
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Correspondence to Jianhua Huang or Hirofumi Hamada.

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Huang, J., Inoue, M., Hasegawa, M. et al. Sendai viral vector mediated angiopoietin-1 gene transfer for experimental ischemic limb disease. Angiogenesis 12, 243–249 (2009). https://doi.org/10.1007/s10456-009-9144-6

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  • DOI: https://doi.org/10.1007/s10456-009-9144-6

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