Abstract
The data of adsorption kinetics of F-specific RNA bacteriophages Qβ and GA on drinking water biofilms under hydrostatic conditions was modeled. The rate limitation of virus adsorption was shown to be the free diffusion in water for GA where as another rate limiting step was demonstrated for Qβ. Modeling results also showed that the number of adsorbed viruses can be fitted with a limitless equation in static conditions. However sorption–desorption assays carried out in dynamic conditions showed that Qβ and GA phages have a similar affinity for the biofilm and reinforced that no significant virus desorption occurred during the first 10 h of adsorption from a bulk containing virus in static conditions. The small surface properties variations between the two phages do not induce significant differences of their adsorbed quantities in hydrodynamic conditions but they significantly affect the rate at which adsorption occurs in hydrostatic conditions.
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Abbreviations
- A:
-
Surface area of the reactor in contact with water
- D:
-
Diffusion coefficient of the viruses in water
- HDPE:
-
High density polyethylene
- Kads :
-
Adsorption constant
- Nads :
-
Number of viruses adsorbed on the overall surface area of the reactor
- Nads.eq :
-
Number of viruses adsorbed on the surface area of the reactor at equilibrium
- \(\tilde{N}_{ads}\) :
-
Number of viruses adsorbed normalized by the N0, V and A
- N0 :
-
Number of viruses introduced in the system
- PFU:
-
Plaque Forming Units
- t:
-
Time
- V:
-
Overall volume of the reactor
- α0 :
-
Statistic factor taking into account the efficiency of the contact between the viruses and the surface
- β:
-
Characteristic time of site occupancy
- δ0 :
-
Distance parameter allowing potential initial adsorption (at experimental t = 0) to be taken into account
- δ1 :
-
Diffusion layer thickness parameter allowing a potential kinetic limitation of the flux to be taken into account
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Acknowledgments
The results of this work were obtained within the scope of a study (Biofilm VIII) supported by the following partners: Syndicat des Eauxd’Ile-de-France (SEDIF), VERI (VEOLIA Environnement Rechercheet Innovation), and Agence de l’Eau Seine Normandie, under the coordination provided by the Université de Lorraine (Nancy, F).
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Hébrant, M., Pelleïeux, S., Mathieu, L. et al. Distinct adsorption kinetics of Qβ and GA bacteriophages on drinking water biofilms. Adsorption 20, 823–828 (2014). https://doi.org/10.1007/s10450-014-9624-x
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DOI: https://doi.org/10.1007/s10450-014-9624-x