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Nanoliposomal Growth Hormone and Sodium Nitrite Release from Silicone Fibers Reduces Thrombus Formation Under Flow

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Abstract

Biocompatibility of artificial lungs can be improved by endothelialization of hollow fibers. Bioavailability of growth-inducing and anti-thrombotic agents on the hollow fiber–blood interface inhibits thrombosis. We investigated if nanoliposomal growth-inducing growth hormone (nGH) and anti-thrombotic sodium nitrite (nNitrite) incorporation into collagen-coating on silicone hollow fibers improves blood biocompatibility by increasing endothelial cell growth and nitrite bioavailability under flow. Nitrite production rate was assessed under varying flow conditions. Finite element (FE) modeling was used to simulate nitrite transport within the parallel-plate flow chamber, and nitrite bioavailability on the fiber–blood interface at 1–30 dyn/cm2 shear stress. Endothelial cell number on fibers coated with nNitrite-nGH-collagen conjugate was 1.5-fold higher than on collagen-coated fibers. For collagen-coated fibers, nitrite production reached a maximum at 18 dyn/cm2 shear stress. When fibers were coated with nNitrite-nGH-collagen conjugate, nitrite production increased continuously by increasing shear stress. FE modeling revealed that nitrite concentrations at the fiber–blood interface were affected by shear stress-induced nitrite production, and diffusion/convection-induced nitrite removal. Highest nitrite concentrations and lowest thrombus deposition were observed on fibers coated with nNitrite-nGH-collagen conjugate exposed to 6–12 dyn/cm2 shear stress. In conclusion, our results suggest that nNitrite-nGH-Col conjugate coatings promote endothelialization of silicone hollow fibers in biohybrid artificial lungs.

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Acknowledgments

The authors are thankful to the scientific support of members of the Pasteur Institute of Iran, in particular the help of Prof. Mohammad Ali Shokrgozar.

Conflict of interest

There is no conflict of interests.

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Authors and Affiliations

  1. School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11365-4563, Enqelab Avenue, Tehran, Iran

    Nasim Salehi-Nik, Ghassem Amoabediny & Seyedeh Parnian Banikarimi

  2. Research Center for New Technologies in Life Science Engineering, University of Tehran, 143951374, Enqelab Avenue, Tehran, Iran

    Nasim Salehi-Nik, Ghassem Amoabediny, Seyedeh Parnian Banikarimi & Zahra Malaie-Balasi

  3. Department of Orthopedics, University Medical Center Utrecht, Heidelberglaan 100, 3584 CX, Utrecht, The Netherlands

    Behdad Pouran

  4. Department of Biomechanical Engineering, Faculty of Mechanical, Maritime, and Materials Engineering, Delft University of Technology, Mekelweg 2, 2628 CD, Delft, The Netherlands

    Behdad Pouran

  5. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands

    Behrouz Zandieh-Doulabi & Jenneke Klein-Nulend

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  1. Nasim Salehi-Nik
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Correspondence to Ghassem Amoabediny.

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Associate Editor Peter E. McHugh oversaw the review of this article.

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Salehi-Nik, N., Amoabediny, G., Banikarimi, S.P. et al. Nanoliposomal Growth Hormone and Sodium Nitrite Release from Silicone Fibers Reduces Thrombus Formation Under Flow. Ann Biomed Eng 44, 2417–2430 (2016). https://doi.org/10.1007/s10439-015-1529-7

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  • DOI: https://doi.org/10.1007/s10439-015-1529-7

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