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Discovery of Retinal Elastin and Its Possible Role in Age-Related Macular Degeneration

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Abstract

Age-related macular degeneration (AMD) etiology is unknown, but its association to atherosclerotic vascular disease (ASVD) has been observed. Since elastin plays an important role in the atherosclerotic process, to understand ASVD and AMD’s relationship we examined retinal elastin existence, elastin amount and vessel properties among normal subjects, mild AMD patients, moderate-to-severe AMD patients, and ASVD patients (n = 20). One eye per donor was assigned to enzyme-linked immunosorbent assay for quantifying the retinal elastin amount. The rest were assigned to mechanical test for examining the retinal vessel properties. Additionally, two normal human and two porcine eyes were acquired in immunohistochemistry for locating the retinal elastin. We found that elastin presented in the human and porcine retinal vessels at the basement membranes. 3.73 ± 0.55% of the normal retinal tissues were elastin. Elastin decrease, tissue-weight increase, and vessel hardening and inelasticity (p < 0.05) were observed in the retina of patients with ASVD and only moderate-to-severe (i.e., not mild) AMD. Most moderate-to-severe AMD patients also happened to have ASVD. The results suggest that ASVD is unlikely the cause of AMD, but it is perhaps a factor that aggravates the condition through mechanism associated with retinal vessel abnormality.

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References

  1. Armstrong, M. L., and M. B. Megan. Arterial fibrous proteins in cynomolgus monkeys after atherogenic and regression diets. Circ. Res. 36:256–261, 1975.

    Article  CAS  PubMed  Google Scholar 

  2. Åstrand, H., J. Stålhand, J. Karlsson, M. Karlsson, B. Sonesson, and T. Länne. In vivo estimation of the contribution of elastin and collagen to the mechanical properties in the human abdominal aorta: effect of age and sex. J. Appl. Physiol. 110:176–187, 2011.

    Article  PubMed  Google Scholar 

  3. Bernstein, E. F., Y. Q. Chen, K. Tamai, K. J. Shepley, K. S. Resnik, H. Zhang, R. Tuan, A. Mauviel, and J. Uitto. Enhanced elastin and fibrillin gene expression in chronically photodamaged skin. J. Invest. Dermatol. 103:182–186, 1994.

    Article  CAS  PubMed  Google Scholar 

  4. Brooke, B. S., A. Bayes-Genis, and D. Y. Li. New insights into elastin and vascular disease. Trends Cardiovasc. Med. 13:176–181, 2003.

    Article  CAS  PubMed  Google Scholar 

  5. Chen, K., B. Fata, and D. R. Einstein. Characterization of the highly nonlinear and anisotropic vascular tissues from experimental inflation data: a validation study toward the use of clinical data for in vivo modeling and analysis. Ann. Biomed. Eng. 36:1668–1680, 2008.

    Article  PubMed Central  PubMed  Google Scholar 

  6. Chen, K., A. P. Rowley, and J. D. Weiland. Elastic properties of porcine ocular posterior soft tissues. J. Biomed. Mater. Res. A 93:634–645, 2010.

    PubMed  Google Scholar 

  7. Chen, K., A. P., J. D. Weiland, and M. S. Humayun. Elastic properties of human posterior eye. J. Biomed. Mater. Res. A. DOI:10.1002/jbm.a.34858.

  8. Chen, K., and J. D. Weiland. Anisotropic and inhomogeneous mechanical characteristics of the retina. J. Biomech. 43:1417–1421, 2010.

    Article  PubMed  Google Scholar 

  9. Daga-Gordini, D., G. M. Bressan, I. Castellani, and D. Volpin. Detection of elastin by immunoelectronmicroscopy. Histochemistry 87:573–578, 1987.

    Article  CAS  PubMed  Google Scholar 

  10. Debelle, L., and A. M. Tamburro. Elastin: molecular description and function. Int. J. Biochem. Cell Biol. 31:261–272, 1999.

    Article  CAS  PubMed  Google Scholar 

  11. Ditzel, J. The problem of tissue oxygenation in diabetes mellitus. Acta Med. Scand. 578:59–68, 1975.

    CAS  Google Scholar 

  12. Eye Conditions: Macular Degeneration. Lasik Surgery Cost, 2012. http://lasiksurgerycost.net/macular-degeneration. Accessed 28 May 2012.

  13. Faris, B., P. Toselli, J. Kispert, B. L. Wolfe, C. A. Pratt, P. J. Mogayzel, Jr., and C. Franzblau. Elastase effect on the extracellular matrix of rat aortic smooth muscle cells in culture. Exp. Mol. Pathol. 45:105–117, 1986.

    Article  CAS  PubMed  Google Scholar 

  14. Friedman, E. The role of the atherosclerotic process in the pathogenesis of age-related macular degeneration. Am. J. Ophthalmol. 130:658–663, 2000.

    Article  CAS  PubMed  Google Scholar 

  15. Gerli, R., L. Ibba, and C. Fruschelli. Ultrastructural cytochemistry of anchoring filaments of human lymphatic capillaries and their relation to elastic fibers. Lymphology 24:105–112, 1991.

    CAS  PubMed  Google Scholar 

  16. Giannoudis, P. V., H. Dinopoulos, B. Chalidis, and G. M. Hall. Surgical stress response. Injury 37:s3–s9, 2006.

    Article  PubMed  Google Scholar 

  17. Gosline, J., M. Lillie, E. Carrington, P. Guerette, C. Ortlepp, and K. Savage. Elastic proteins: biological roles and mechanical properties. Philos. Trans. R. Soc. Lond. B 357:121–132, 2002.

    Article  CAS  Google Scholar 

  18. Hirvelä, H., H. Luukinen, E. Läärä, L. Sc, and L. Laatikainen. Risk factors of age-related maculopathy in a population 70 years of age or older. Ophthalmology 103:871–877, 1996.

    Article  PubMed  Google Scholar 

  19. Hogan, M. J., J. A. Alvarado, and J. E. Weddell. Histology of the Human Eye: an Atalas and Textbook. Philadelphia: WB Saunders, 1971.

    Google Scholar 

  20. Hogan, M. J., and L. Feeney. The ultrastructure of the retinal blood vessels: I. The large vessels. J. Ultrastruct. Res 39:10–28, 1963.

    Article  CAS  PubMed  Google Scholar 

  21. Klein, R., T. Peto, A. Bird, and M. Vannewkirk. The epidemiology of age-related macular degeneration. Am. J. Ophthalmol. 137:486–495, 2004.

    Article  PubMed  Google Scholar 

  22. MacSweeney, S. T. R., G. Young, R. M. Greenhalgh, and J. T. Powell. Mechanical properties of the aneurysmal aorta. Br. J. Surg. 79:1281–1284, 1992.

    Article  CAS  PubMed  Google Scholar 

  23. Mecham, R. P., J. Madaras, J. A. McDonald, and U. Ryan. Elastin production by cultured calf pulmonary artery endothelial cells. J. Cell. Physiol. 116:282–288, 1983.

    Article  CAS  PubMed  Google Scholar 

  24. Mullins, R. F., S. R. Russell, D. H. Anderson, and G. S. Hageman. Drusen associated with aging and age-related macular degeneration contain proteins common to extracellular deposits associated with atherosclerosis, elastosis, amyloidosis, and dense deposit disease. FASEB J. 14:835–846, 2000.

    CAS  PubMed  Google Scholar 

  25. Murray, C. J., and A. D. Lopez. Alternative projections of mortality and disability by cause 1990–2020: global burden of disease study. Lancet 349:1498–1504, 1997.

    Article  CAS  PubMed  Google Scholar 

  26. Neuman, R. E., and M. A. Logan. The determination of collagen and elastin in tissues. J. Biol. Chem. 186:549–556, 1995.

    Google Scholar 

  27. Robert, L., A. M. Robert, and B. Jacotot. Elastin-elastase-atherosclerosis revisited. Atherosclerosis 140:281–295, 1998.

    Article  CAS  PubMed  Google Scholar 

  28. Sagaties, M. J., and G. Raviola. Immunogold staining of elastin in the wall of the retinal arteries in Macaca mulatta. Curr. Eye Res. 8:55–59, 1988.

    Article  Google Scholar 

  29. Seifert, P., and W. Sekundo. Capillaries in the epithelium of pterygium. Br. J. Ophthalmol. 82:77–81, 1998.

    Article  CAS  PubMed  Google Scholar 

  30. Sivaprasad, S. Investigations of a Possible Link Between Age-Related Macular Degeneration and Atherosclerosis. Bedforshire: Cranfield University, 2005.

    Google Scholar 

  31. Vingerling, J. R., I. Dielemans, M. L. Bots, A. Hofman, D. E. Grobbee, and P. T. V. M. de Jong. Age-related macular degeneration is associated with atherosclerosis. Am. J. Epidemiol. 142:404–409, 1995.

    CAS  PubMed  Google Scholar 

  32. Wise, G. N., C. T. Dollery, and P. Henkind. The Retinal Circulation. New York: Harper & Row, 1971.

    Google Scholar 

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Acknowledgments

This work is financially supported by the Department of Energy’s Office of Science (grant no. DE-FC02-04ER63735), W.M. Keck Foundation, and Clarence and Estelle Albaugh Trust. The authors would like to thank Ruth Phinney, Xiao Peng Wang, Douglas Hauser, and Ronald Pak Cheung Wong for their technical assistance on donor coordination, sectioning, electron microscope operation, and ELISA.

Conflict of interest

The authors have no conflict of interest, financial or otherwise with this work.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Southern California – Denney Research Center 140, 1042 Downey Way, Los Angeles, CA, 90089, USA

    Kinon Chen & James D. Weiland

  2. Department of Ophthalmology, University of Southern California – Doheny Eye Institute, 1450 San Pablo Street, Los Angeles, CA, 90033, USA

    Kinon Chen & James D. Weiland

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  1. Kinon Chen
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  2. James D. Weiland
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Correspondence to Kinon Chen.

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Associate Editor Holly Ober oversaw the review of this article.

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Chen, K., Weiland, J.D. Discovery of Retinal Elastin and Its Possible Role in Age-Related Macular Degeneration. Ann Biomed Eng 42, 678–684 (2014). https://doi.org/10.1007/s10439-013-0936-x

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  • DOI: https://doi.org/10.1007/s10439-013-0936-x

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