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Gender-Dependence of Bone Structure and Properties in Adult Osteogenesis Imperfecta Murine Model

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Abstract

Osteogenesis imperfecta (OI) is a dominant skeletal disorder characterized by bone fragility and deformities. Though the oim mouse model has been the most widely studied of the OI models, it has only recently been suggested to exhibit gender-dependent differences in bone mineralization. To characterize the impact of gender on the morphometry/ultra-structure, mechanical properties, and biochemical composition of oim bone on the congenic C57BL/J6 background, 4-month-old oim/oim, +/oim, and wild-type (wt) female and male tibiae were evaluated using micro-computed tomography, three-point bending, and Raman spectroscopy. Dramatic gender differences were evident in both cortical and trabecular bone morphological and geometric parameters. Male mice had inherently more bone and increased moment of inertia than genotype-matched female counterparts with corresponding increases in bone biomechanical strength. The primary influence of gender was structure/geometry in bone growth and mechanical properties, whereas the mineral/matrix composition and hydroxyproline content of bone were influenced primarily by the oim collagen mutation. This study provides evidence of the importance of gender in the evaluation and interpretation of potential therapeutic strategies when using mouse models of OI.

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Acknowledgments

The authors gratefully thank Mark Dallas for assistance with μCT and three-point bending experiments. This work was supported by National Institutes of Health grant R01AR055907. This study utilized the Center for Research on Interfacial Structure and Properties (CRISP) and Center of Excellence in Mineralized Tissues (CEMT) facilities at the University of Missouri Kansas City.

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The authors confirm they have full control of all primary data and will allow the journal to review the data. The authors confirm there is no conflict of interest or financial relationship with the organizations that sponsored this research.

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Authors and Affiliations

  1. Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri-Kansas City, 650 E. 25th St., Kansas City, MO, 64108, USA

    Xiaomei Yao, Jennifer Melander & Yong Wang

  2. Department of Biochemistry, University of Missouri-Columbia, 117 Schweitzer Hall, Columbia, MO, 65211, USA

    Stephanie M. Carleton, Arin D. Kettle & Charlotte L. Phillips

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  1. Xiaomei Yao
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  2. Stephanie M. Carleton
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  3. Arin D. Kettle
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  4. Jennifer Melander
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  5. Charlotte L. Phillips
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  6. Yong Wang
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Correspondence to Charlotte L. Phillips or Yong Wang.

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Associate Editor Mona Kamal Marei oversaw the review of this article.

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Yao, X., Carleton, S.M., Kettle, A.D. et al. Gender-Dependence of Bone Structure and Properties in Adult Osteogenesis Imperfecta Murine Model. Ann Biomed Eng 41, 1139–1149 (2013). https://doi.org/10.1007/s10439-013-0793-7

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