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Modulation of Hyaluronan Fragmentation by Interleukin-1 Beta in Synovial Membrane Cells

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Abstract

Hyaluronan (HA) plays a crucial role in the lubricating and buffering properties of synovial fluid. The purpose of this study was to examine the effects of interleukin (IL)-1β on HA degradation in cultured synovial membrane cells. The rabbit synovial membrane cell line HIG-82 was cultured with and without IL-1β. The amounts of HA of varying molecular weights in the medium were analyzed using high-performance liquid chromatography, the mRNA levels of HA synthase (HAS) and hyaluronidase (HYAL) were analyzed by means of real-time PCR, and HYAL activity was analyzed by HA zymography. The amounts of HA with a molecular weight lower than 300 kDa, and between 300 and 1900 kDa, in the culture medium of HIG-82 cells were significantly higher in the presence of IL-1β. However, the amount of HA with a molecular weight greater than 1900 kDa was significantly lower in the presence of IL-1β. Both HAS2 and HAS3 mRNA levels were upregulated by treatment with IL-1β. So, too, were the levels of HYAL1 and HYAL2 mRNA, which resulted in enhanced HYAL activity. However, HYAL activity was inhibited by transfection of HYAL2-siRNA. Our results suggest that IL-1β is a crucial factor in the fragmentation of HA in inflammatory joints.

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Acknowledgment

This research was supported by a Grant-in-Aid (No. 19659540) for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan

    K. Tanimoto, T. Yanagida, Y. Tanne, T. Kamiya, Y. C. Huang, T. Mitsuyoshi, N. Tanaka & K. Tanne

  2. Department of Orthodontics and Dentofacial Orthopedics, The University of Tokushima Graduate School of Oral Sciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8504, Japan

    E. Tanaka

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  1. K. Tanimoto
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  2. T. Yanagida
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Correspondence to K. Tanimoto.

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Associate Editor Ceng Dong oversaw the review of this article.

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Tanimoto, K., Yanagida, T., Tanne, Y. et al. Modulation of Hyaluronan Fragmentation by Interleukin-1 Beta in Synovial Membrane Cells. Ann Biomed Eng 38, 1618–1625 (2010). https://doi.org/10.1007/s10439-010-9927-3

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  • DOI: https://doi.org/10.1007/s10439-010-9927-3

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