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Multifunctional FePt Nanoparticles for Radiation-Guided Targeting and Imaging of Cancer

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Abstract

A multifunctional FePt nanoparticle was developed that targets tumor microvasculature via “radiation-guided” peptides, and is detected by both near-infrared (NIR) fluorescence imaging and analytical mass spectrometry methods. Tumor specific binding was first measured by biotinylated peptide linked to fluorophore-conjugated streptavidin. This showed tumor selective binding to tumors using the HVGGSSV peptide. FePt nanoparticles were synthesized sequentially by surface modification with poly(l)lysine, poly(ethylene) glycol conjugation, and functionalized with HVGGSSV peptide and fluorescent probe Alexa fluor 750. NIR fluorescence imaging and ICP-MS analysis showed significant HVGGSSV-FePt nanoparticle binding to irradiated tumors as compared to unirradiated tumors and controls. Results indicate that multifunctional FePt nanoparticles have potential application for radiation-guided targeting and imaging of cancer.

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Acknowledgments

This work was supported by Vanderbilt-Ingram Cancer Center, and NIH grants P50CA128323, R01-CA112385, R21-CA128456, and R01-CA125757. We thank Rossane Delapp for technical assistance.

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Authors and Affiliations

  1. Department of Chemistry, Vanderbilt University, Nashville, TN, 37235, USA

    Ghazal Hariri, Matthew S. Wellons, William H. Morris III & Charles M. Lukehart

  2. Department of Radiation Oncology, Washington University School of Medicine, 4511 Forest Park, St. Louis, MO, 63108, USA

    Dennis E. Hallahan

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  1. Ghazal Hariri
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  2. Matthew S. Wellons
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  3. William H. Morris III
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  4. Charles M. Lukehart
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  5. Dennis E. Hallahan
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Corresponding author

Correspondence to Dennis E. Hallahan.

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Associate Editor Konstantinos Konstantopoulos oversaw the review of this article.

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Hariri, G., Wellons, M.S., Morris, W.H. et al. Multifunctional FePt Nanoparticles for Radiation-Guided Targeting and Imaging of Cancer. Ann Biomed Eng 39, 946–952 (2011). https://doi.org/10.1007/s10439-010-0219-8

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  • DOI: https://doi.org/10.1007/s10439-010-0219-8

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