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A droplet-generator-on-a-chip actuated by ECF (electro-conjugate fluid) micropumps

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Abstract

The traditional power sources for generating droplets are bulky devices such as syringe pumps, constant pressure systems and so on, resulting in difficult integration with other microfluidic components. To overcome this limitation, we propose and develop a droplet generator with its power sources on a chip (22 × 21 × ~ 1 mm). The compact droplet generator consists of two ECF (electro-conjugate fluid) micropumps, a T-junction channel geometry made of SU-8, a water chamber and three I/O ports. In our methodology, the two ECF micropumps are used to pump the continuous phase fluid (oil) directly and the dispersed phase fluid (water) indirectly on a chip. ECF is a kind of functional and dielectric oil, which can generate a strong and active ECF jet when its corresponding electrodes are applied to high DC voltages. Our ECF micropump is composed of triangular prism and silt electrode pairs (TPSEs) and is able to control flow rates precisely on a chip. In our device, the water in oil droplet is formed by the T-junction geometry. Using MEMS technology, we successfully fabricate the droplet-generator-on-a-chip. Span 80 and dibutyl decanedioate (DBD, Tokyo Chemical Industry Co., Ltd) are used as the surfactant and one type of ECF, respectively. Prior to the characteristic experiments of the T-junction generator, we investigate viscosity, electrical conductivity and relative permittivity of the mixtures of DBD and Span 80 at different concentrations (0 wt%, 1 wt%, 3 wt% and 5 wt%). We also research the impact of the mixtures on the performance of ECF micropumps and the wettability of SU-8 fluidic channels. After that, we confirm the flow pattern diagrams of two-phase fluids, the generated droplet diameter and the droplet production rate for our droplet-generator. Therefore, our droplet generator powered by ECF micropumps can realize the droplet generation on a chip.

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References

  • Abate AR, Weitz DA (2011) Syringe-vacuum microfluidics: a portable technique to create monodisperse emulsions. Biomicrofluidics 5:014107

    Google Scholar 

  • Abate AR, Mary P, van Steijn V, Weitz DA (2012a) Experimental validation of plugging during drop formation in a T-junction. Lab Chip 12:1516–1521

    Google Scholar 

  • Abate AR, Mary P, van Steijn V, Weitz DA (2012b) Experimental validation of plugging during drop formation in a T-junction. Lab Chip 12:1516–1521

    Google Scholar 

  • Baret J-C (2012) Surfactants in droplet-based microfluidics. Lab Chip 12:422–433

    Google Scholar 

  • Bashir S, Bashir M, Rees JM, Zimmerman WBJ (2014) Dynamic wetting in microfluidic droplet formation. BioChip J 8:122–128

    Google Scholar 

  • Berry JD, Neeson MJ, Dagastine RR, Chan DY, Tabor RF (2015) Measurement of surface and interfacial tension using pendant drop tensiometry. J Colloid Interface Sci 454:226–237

    Google Scholar 

  • Dangla R, Gallaire F, Baroud CN (2010) Microchannel deformations due to solvent-induced PDMS swelling. Lab Chip 10:2972–2978

    Google Scholar 

  • Dukhin AS, Goetz PJ (2006) How non-ionic “electrically neutral” surfactants enhance electrical conductivity and ion stability in non-polar liquids. J Electroanal Chem 588:44–50

    Google Scholar 

  • Ekwall B, Nordensten C, Albanus L (1982) Toxicity of 29 plasticizers to HeLa cells in the MIT-24 system. Toxicology 24:199–210

    Google Scholar 

  • Galas J-C, Bartolo D, Studer V (2009) Active connectors for microfluidic drops on demand. New J Phys 11:075027

    Google Scholar 

  • García-Sánchez P, Ferney M, Ren Y, Ramos A (2012) Actuation of co-flowing electrolytes in a microfluidic system by microelectrode arrays. Microfluid Nanofluid 13:441–449

    Google Scholar 

  • http://www.newtech-ecf.com/html/prof_e_s.htm

  • Iwai K, Shih KC, Lin X, Brubaker TA, Social RD, Lin L (2014) Finger-powered microfluidic systems using multilayer soft lithography and injection molding processes. Lab Chip 14:3790–3799

    Google Scholar 

  • Kang X, Luo C, Wei Q, Xiong C, Chen Q, Chen Y, Ouyang Q (2013) Mass production of highly monodisperse polymeric nanoparticles by parallel flow focusing system. Microfluid Nanofluid 15:337–345

    Google Scholar 

  • Kim J-W et al (2012) Tube-type micropump by using electro-conjugated fluid (ECF). Sens Actuators A 174:155–161

    Google Scholar 

  • Kim J-w, Tanabe Y, Yokota S (2019) Comprehending electro-conjugate fluid (ECF) Jets by using the onsager effect. Sens Actuators A 295:266–273

    Google Scholar 

  • Kobayashi I, Wada Y, Uemura K, Nakajima M (2010) Microchannel emulsification for mass production of uniform fine droplets: integration of microchannel arrays on a chip. Microfluid Nanofluid 8:255–262

    Google Scholar 

  • Kuroboshi Y, Takemura K, Edamura K (2018) Understanding of electro-conjugate fluid flow with dibutyl decanedioate using numerical simulation—Calculating ion mobility using molecular dynamics simulation. Sens Actuators B Chem 255:448–453

    Google Scholar 

  • Lan W, Li S, Xu J, Luo G (2012) A one-step microfluidic approach for controllable preparation of nanoparticle-coated patchy microparticles. Microfluid Nanofluid 13:491–498

    Google Scholar 

  • Lim J, Caen O, Vrignon J, Konrad M, Taly V, Baret J-C (2015) Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Biomicrofluidics 9:034101

    Google Scholar 

  • Lin B-C, Su Y-C (2008) On-demand liquid-in-liquid droplet metering and fusion utilizing pneumatically actuated membrane valves. J Micromech Microeng 18:115005

    Google Scholar 

  • Lippold BC, Gunder W, Lippold BH (1999) Drug release from diffusion pellets coated with the aqueous ethylcellulose dispersion aquacoat® ECD-30 and 20% dibutyl sebacate as plasticizer: partition mechanism and pore diffusion. Eur J Pharm Biopharm 47:27–32

    Google Scholar 

  • Lorenz H, Despont M, Fahrni N, LaBianca N, Renaud P, Vettiger P (1997) SU-8: a low-cost negative resist for MEMS. J Micromech Microeng 7:121

    Google Scholar 

  • Man J, Li Z, Li J, Chen H (2017) Phase inversion of slug flow on step surface to form high viscosity droplets in microchannel. Appl Phys Lett 110:181601

    Google Scholar 

  • Mao Z, Yoshida K, Kim J-w (2019a) Developing O/O (oil-in-oil) droplet generators on a chip by using ECF (electro-conjugate fluid) micropumps. Sens Actuators B Chem 7:126669

    Google Scholar 

  • Mao Z, Yoshida K, Kim J-w (2019b) Releasing large-area SU-8 structures without using any sacrificial layers. Microelectron Eng 212:53–60

    Google Scholar 

  • Mao Z, Yoshida K, Kim J-w (2019c) A micro vertically-allocated SU-8 check valve and its characteristics. Microsyst Technol 25:245–255

    Google Scholar 

  • Mao Z, Yoshida K, Kim J-w (2019d) Fast packaging by a partially-crosslinked SU-8 adhesive tape for microfluidic sensors and actuators. Sens Actuators A Phys 289:77–86

    Google Scholar 

  • Martinez-Duarte R, Madou M (2011) SU-8 photolithography and its impact on microfluidics. Microfluid Nanofluid Handb 2011:231–268

    Google Scholar 

  • Matsubara T, Huyn HH, Yoshida K, Kim J-w (2019) Development of MEMS-fabricated bidirectional ECF (electro-conjugate fluid) micropumps. Sens Actuators A 295:317–323

    Google Scholar 

  • Murthy N, Xu M, Schuck S, Kunisawa J, Shastri N, Fréchet JM (2003) A macromolecular delivery vehicle for protein-based vaccines: acid-degradable protein-loaded microgels. Proc Natl Acad Sci 100:4995–5000

    Google Scholar 

  • Nagaoka T, Mao Z, Takemura K, Yokota S, Kim JW (2019) ECF (electro-conjugate fluid) finger with bidirectional motion and its application to a flexible hand. Smart Mater Struct 28:025032

    Google Scholar 

  • Nakashoji Y, Tanaka H, Tsukagoshi K, Hashimoto M (2017) A poly (dimethylsiloxane) microfluidic sheet reversibly adhered on a glass plate for the creation of emulsion droplets for droplet digital PCR. Electrophoresis 38:296–304

    Google Scholar 

  • Ober MS et al (2018) Development of biphasic formulations for use in electrowetting-based liquid lenses with a high refractive index difference ACS combinatorial science 20:554–566

    Google Scholar 

  • Okura N, Nakashoji Y, Koshirogane T, Kondo M, Tanaka Y, Inoue K, Hashimoto M (2017) A compact and facile microfluidic droplet creation device using a piezoelectric diaphragm micropump for droplet digital PCR platforms. Electrophoresis 38:26966

    Google Scholar 

  • Pereira Mavis C. “Cosmetic composition.” U.S. Patent No. 4,981,845. 1 Jan 1991

  • Raghavan R, Qin J, Yeo LY, Friend JR, Takemura K, Yokota S, Edamura K (2009) Electrokinetic actuation of low conductivity dielectric liquids. Sens Actuators B Chem 140:287–294

    Google Scholar 

  • Teste B, Jamond N, Ferraro D, Viovy J-L, Malaquin L (2015) Selective handling of droplets in a microfluidic device using magnetic rails. Microfluid Nanofluid 19:141–153

    Google Scholar 

  • Tice JD, Lyon AD, Ismagilov RF (2004) Effects of viscosity on droplet formation and mixing in microfluidic channels. Anal Chim Acta 507:73–77

    Google Scholar 

  • Vaidyanathan R, Dey S, Carrascosa LG, Shiddiky MJ, Trau M (2015) Alternating current electrohydrodynamics in microsystems: pushing biomolecules and cells around on surfaces. Biomicrofluidics 9:061501

    Google Scholar 

  • Wang Y-N, Fu L-M (2018) Micropumps, and biomedical applications: a review. Microelectron Eng 195:121–138

    Google Scholar 

  • Xu J, Li S, Tan J, Wang Y, Luo G (2006) Controllable preparation of monodisperse O/W and W/O emulsions in the same microfluidic device. Langmuir 22:7943–7946

    Google Scholar 

  • Yang C-G, Liu Y-H, Di Y-Q, Xu Z-R (2015) Generation of two-dimensional concentration-gradient droplet arrays on a two-layer chip for the screening of protein crystallization conditions. Microfluid Nanofluid 18:493–501

    Google Scholar 

  • Yannai S (2003) Dictionary of food compounds with CD-ROM: additives, flavors, and ingredients. CRC, London

    Google Scholar 

  • Yokota S (2014) A review on micropumps from the viewpoint of volumetric power density. Mech Eng Rev 1:DSM0014

    Google Scholar 

  • Yokota, Shinichi, Yasufumi Otsubo, and Kazuya Edamura. “Method of using electro-sensitive movable fluids.” U.S. Patent No. 6,495,071. 17 Dec 2002

  • Yuan Y, Lee TR (2013) Contact angle, and wetting properties. In: Surface science techniques. Springer, Berlin, pp 3–34

    Google Scholar 

  • Zhao B, Cui X, Ren W, Xu F, Liu M, Ye Z-G (2017) A controllable and integrated pump-enabled microfluidic chip and its application in droplets generating. Sci Rep 7:11319

    Google Scholar 

  • Zhu P, Wang L (2017) Passive and active droplet generation with microfluidics: a review. Lab Chip 17:34–75

    Google Scholar 

Download references

Acknowledgements

A part of this work was supported by a JSPS KAKENHI Grant-in-Aid for Scientific Research (B), Grant number 18H01359.

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Correspondence to Joon-wan Kim.

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Mao, Z., Yoshida, K. & Kim, Jw. A droplet-generator-on-a-chip actuated by ECF (electro-conjugate fluid) micropumps. Microfluid Nanofluid 23, 130 (2019). https://doi.org/10.1007/s10404-019-2298-7

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