Abstract
This article reports a microfluidic device for the generation of stable, steady-state, user-defined concentration profiles for the long-term maintenance of stable diffusion gradients. The microinstrument allows both dynamic temporal and dynamic spatial control over user-defined concentrations and concentration gradients of multiple chemicals. With this device, one can create an in vitro environment capable of approximating the complex in vivo biological condition for cellular studies. In addition, the device has potential application in combinatory drug discovery, electrophoretic applications, ligand binding, etc. 3D computer simulations and analysis of arbitrary concentration profiles are presented along with experimental validation using multiple diffusing species.
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Smith, R.L., Demers, C.J. & Collins, S.D. Microfluidic device for the combinatorial application and maintenance of dynamically imposed diffusional gradients. Microfluid Nanofluid 9, 613–622 (2010). https://doi.org/10.1007/s10404-010-0574-7
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DOI: https://doi.org/10.1007/s10404-010-0574-7