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Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand

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Abstract

In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of small mammal species was carried out at seven main sites in the region (Cambodia, Lao People’s Democratic Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia). Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites. Antibody prevalence at each site varied from 0 to 5.6% with antibodies detected in several rodent species (Bandicota indica, B. savilei, Maxomys surifer, Mus caroli, M. cookii, Rattus exulans, R. nitidius, R. norvegicus, and R. tanezumi). When site seroprevalence was compared with site species richness, seropositive animals were found more frequently at sites with lower species richness. In order to confirm which hantavirus species were present, a subset of samples was also subjected to RT-PCR. Hantaviral RNA was detected at a single site from each country. Sequencing confirmed the presence of two hantavirus species, Thailand and Seoul viruses, including one sample (from Lao PDR) representing a highly divergent strain of Seoul virus. This is the first molecular evidence of hantavirus in Lao PDR and the first reported L segment sequence data for Thailand virus.

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Acknowledgments

The authors wish to thank Mr. Hul Vibol and Mr. Y Bunthin for their technical assistance in both the laboratory and the field and to Mr. Kim Aun for his assistance in the field. Also to Maria Walhström for kindly preparing the IFA slides used in this study. The CERoPath project (Community ecology of rodents and their pathogens in South East Asia: effects of biodiversity changes and implications in health ecology/ANR 07 BDIV 012) is funded by the Agence Nationale de la Recherche (ANR, France).

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Authors and Affiliations

  1. Virology Unit, Institut Pasteur du Cambodge, 5 Monivong Boulevard, BP 983, Phnom Penh, Cambodia

    Kim Blasdell & Philippe Buchy

  2. Institut des Sciences de l’Evolution, CNRS, IRD, Université Montpellier 2, CC065, 34095, Montpellier Cedex 05, France

    Kim Blasdell & Serge Morand

  3. INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus International de Baillarguet, CS 30016, 34988, Montferrier sur Lez, France

    Jean François Cosson

  4. Centre de Biologie et de Gestion et des Populations (CBGP), International de Baillarguet, CS 30016, 34988, Montferrier sur lez, France

    Yannick Chaval

  5. CIRAD, UR AGIRs (Animal et Gestion Intégrée des Risques), Campus International de Baillarguet, Montpellier, France

    Vincent Herbreteau

  6. National Agricultural Research Centre, National Agricultural and Forestry Research Institute, Vientiane, Lao PDR

    Bounneuang Douangboupha

  7. Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand

    Sathaporn Jittapalapong

  8. Swedish Institute for Infectious Disease Control, Stockholm, Sweden

    Ake Lundqvist

  9. Origine, Structure et Evolution de la Biodiversite, Museum National d’Histoire Naturelle, Paris, France

    Jean-Pierre Hugot

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  1. Kim Blasdell
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  2. Jean François Cosson
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  3. Yannick Chaval
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  10. Philippe Buchy
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Correspondence to Philippe Buchy.

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Blasdell, K., Cosson, J.F., Chaval, Y. et al. Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand. EcoHealth 8, 432–443 (2011). https://doi.org/10.1007/s10393-011-0725-7

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  • DOI: https://doi.org/10.1007/s10393-011-0725-7

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