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Role of IGFBP4 and IGF-I expression in esophageal squamous cell carcinoma

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Abstract

Background

The purpose of this research is to determine the correlation between IGFBP-4/IGF-I expression and the clinical status of esophageal squamous cell carcinoma (ESCC).

Materials and methods

Immunohistochemical staining for IGFBP-4 and IGF-I was performed in 61 clinical samples. The correlation between IGFBP-4/IGF-I staining and clinicopathological features was examined statistically. In addition, we compared the mRNA expression levels of IGFBP-4 and IGF-I between ESCC cancer tissues and the normal esophageal epithelium using a quantitative real-time RT-PCR.

Results

According to the results of immunohistochemical staining, IGFBP-4 expression in the region of normal epithelial cells showed an increase of 52.5 % compared to expression in the tumor cells. There was no statistically significant correlation between IGFBP-4/IGF-I and clinicopathological features. The correlation between IGF-I and IGFBP-4 was not significant either. RT-PCR assay showed IGF-I expression in ESCC tissue has a tendency to be higher than that of normal esophageal epithelium. On the other hand, IGFBP-4 expression levels in cancer tissue were lower in comparison with normal epithelium. These results were not significant.

Conclusions

There were the differences of IGFBP-4 expression between the region of ESCC and the region of normal epithelium cells. One suggested possibility is that IGF-independent action of IGFBP-4 might have taken part in these results. We need further exploratory experiments to elucidate the details of the actual function of IGFBP-4 expression in ESCC.

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Conflict of interest

None declared.

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Authors and Affiliations

  1. Department of Frontier Surgery, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba, 260-8670, Japan

    Yuka Isozaki, Isamu Hoshino, Yasunori Akutsu, Aki Akimoto, Naoki Akanuma, Nobuyoshi Takeshita, Tetsuro Maruyama & Hisahiro Matsubara

Authors
  1. Yuka Isozaki
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  2. Isamu Hoshino
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  3. Yasunori Akutsu
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  4. Aki Akimoto
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  5. Naoki Akanuma
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  6. Nobuyoshi Takeshita
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  7. Tetsuro Maruyama
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  8. Hisahiro Matsubara
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Correspondence to Isamu Hoshino.

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Isozaki, Y., Hoshino, I., Akutsu, Y. et al. Role of IGFBP4 and IGF-I expression in esophageal squamous cell carcinoma. Esophagus 10, 79–85 (2013). https://doi.org/10.1007/s10388-013-0368-6

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  • DOI: https://doi.org/10.1007/s10388-013-0368-6

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