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Intensity Analysis of Hartmann-Shack Images in Cataractous, Keratoconic, and Normal Eyes to Investigate Light Scattering

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Abstract

Purpose

A clinical investigation of novel methods for evaluating light scattering using a Hartmann-Shack aberrometer.

Methods

Aberrometry was performed on normal eyes (n = 7; patient age, 26.7 ± 2.5 years, mean ± SD), eyes with keratoconus (n = 22; patient age, 26.1 ± 8.1 years), and eyes with cataract (n = 17; patient age, 56.5 ± 16.9 years) using a Hartmann-Shack wavefront aberrometer. We introduced two methods: (1) a contrast method, in which we calculated the inverse of contrast of the local images around 12 spots in a Hartmann-Shack image, and (2) a difference of point spread function (PSF) method, in which we analyzed the difference between the width of the PSF computed with aberration information and the width of the measured PSF, which contains both aberration and light scattering information.

Results

The inverse contrast in cataractous eyes (5.04 ± 3.06 inverse contrast units) was significantly larger than that in normal eyes (1.57 ± 0.56) or keratoconic eyes (1.83 ± 0.79). The difference of PSF in cataractous eyes (81.8 ± 65.2 μm) was also significantly larger than that in normal eyes (9.3 ± 4.3 μm) or keratoconic eyes (30.0 ± 20.1 μm). The inverse contrast and the difference in the PSF were highly correlated (r = 0.89, P < 0.0001).

Conclusions

The two methods introduced here successfully distinguished cataractous eyes from normal and keratoconic eyes. After the results were analyzed by a discriminant analysis, the separation of the three categories proved to be excellent. Jpn J Ophthalmol 2006;50:323–333 © Japanese Ophthalmological Society 2006

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References

  1. R Hess G Woo (1978) ArticleTitleVision through cataracts Invest Ophthalmol Vis Sci 17 428–435 Occurrence Handle640789 Occurrence Handle1:STN:280:DyaE1c7ltVamtQ%3D%3D

    PubMed  CAS  Google Scholar 

  2. JK Ijspeert PWT de Waard TJTP van den Berg PVTM de Jong (1990) ArticleTitleThe intraocular straylight function in 129 healthy volunteers; dependence on angle, age and pigmentation Vision Res 30 699–707 Occurrence Handle2378063 Occurrence Handle1:STN:280:DyaK3czjtlKjsQ%3D%3D Occurrence Handle10.1016/0042-6989(90)90096-4

    Article  PubMed  CAS  Google Scholar 

  3. J Liang B Grimm S Goelz JF Bille (1994) ArticleTitleObjective measurement of wave aberrations of the human eye with the use of a Hartmann-Shack wave-front sensor J Opt Soc Am A Opt Image Sci Vis 11 1949–1957 Occurrence Handle8071736 Occurrence Handle1:STN:280:DyaK2czltFCisA%3D%3D Occurrence Handle10.1364/JOSAA.11.001949

    Article  PubMed  CAS  Google Scholar 

  4. J Liang DR Williams (1997) ArticleTitleAberrations and retinal image quality of the normal human eye J Opt Soc Am A Opt Image Sci Vis 14 2873–2883 Occurrence Handle9379245 Occurrence Handle1:STN:280:DyaK1c%2Fit1emug%3D%3D

    PubMed  CAS  Google Scholar 

  5. SM MacRae DR Williams (2001) ArticleTitleWavefront guided ablation Am J Ophthalmol 132 915–919 Occurrence Handle11730658 Occurrence Handle1:STN:280:DC%2BD3MnosVChsg%3D%3D Occurrence Handle10.1016/S0002-9394(01)01319-8

    Article  PubMed  CAS  Google Scholar 

  6. J Liang DR Williams DT Miller (1997) ArticleTitleSupernormal vision and high-resolution retinal imaging through adaptive optics J Opt Soc Am A Opt Image Sci Vis 14 2884–2892 Occurrence Handle9379246 Occurrence Handle1:STN:280:DyaK1c%2Fit1emuw%3D%3D

    PubMed  CAS  Google Scholar 

  7. A Roorda DR Williams (1999) ArticleTitleThe arrangement of the three cone classes in the living human eye Nature 397 520–522 Occurrence Handle10028967 Occurrence Handle1:CAS:528:DyaK1MXhtlCkur4%3D Occurrence Handle10.1038/17383

    Article  PubMed  CAS  Google Scholar 

  8. N Maeda T Fujikado T Kuroda et al. (2002) ArticleTitleWavefront aberrations measured with Hartmann-Shack sensor in patients with keratoconus Ophthalmology 109 1996–2003 Occurrence Handle12414405 Occurrence Handle10.1016/S0161-6420(02)01279-4

    Article  PubMed  Google Scholar 

  9. T Kuroda T Fujikado N Maeda T Oshika Y Hirohara T Mihashi (2002) ArticleTitleWavefront analysis in eyes with nuclear or cortical cataract Am J Ophthalmol 134 1–9 Occurrence Handle12095801 Occurrence Handle10.1016/S0002-9394(02)01402-2

    Article  PubMed  Google Scholar 

  10. T Kuroda T Fujikado N Maeda T Oshika Y Hirohara T Mihashi (2002) ArticleTitleWavefront analysis of higher-order aberrations in patients with cataract J Cataract Refract Surg 28 438–444 Occurrence Handle11973090 Occurrence Handle10.1016/S0886-3350(01)01176-2

    Article  PubMed  Google Scholar 

  11. S Koh N Maeda T Kuroda et al. (2002) ArticleTitleEffect of tear film break-up on higher-order aberrations measured with wavefront sensor Am J Ophthalmol 134 115–117 Occurrence Handle12095817 Occurrence Handle10.1016/S0002-9394(02)01430-7

    Article  PubMed  Google Scholar 

  12. R Montes-Mico JL Alio G Munoz JJ Perez-Santonja WN Charman (2004) ArticleTitlePostblink changes in total and corneal ocular aberrations Ophthalmology 111 758–767 Occurrence Handle15051210 Occurrence Handle10.1016/j.ophtha.2003.06.027

    Article  PubMed  Google Scholar 

  13. O Hockwin V Dragomirescu H Laser (1982) ArticleTitleMeasurements of lens transparency or its disturbances by densitometric image analysis of Scheimpflug photographs Graefes Arch Clin Exp Ophthalmol 219 255–262 Occurrence Handle7160634 Occurrence Handle1:STN:280:DyaL3s7jtlehtA%3D%3D Occurrence Handle10.1007/BF00231409

    Article  PubMed  CAS  Google Scholar 

  14. LT Chylack SuffixJr JK Wolfe DM Singer et al. (1993) ArticleTitleThe Lens Opacities Classification System III. The Longitudinal Study of Cataract Study Group Arch Ophthalmol 111 831–836 Occurrence Handle8512486

    PubMed  Google Scholar 

  15. J Liang G Westheimer (1995) ArticleTitleOptical performances of human eyes derived from double-pass measurements J Opt Soc Am A Opt Image Sci Vis 12 1411–1416 Occurrence Handle7608785 Occurrence Handle1:STN:280:DyaK2Mzjt12rtg%3D%3D

    PubMed  CAS  Google Scholar 

  16. LN Thibos X Hong (1999) ArticleTitleClinical applications of the Shack-Hartmann aberrometer Optom Vis Sci 76 817–825 Occurrence Handle10612402 Occurrence Handle1:STN:280:DC%2BD3c%2FnslKnuw%3D%3D

    PubMed  CAS  Google Scholar 

  17. WJ Donnelly Suffix3rd K Pesudovs JD Marsack EJ Sarver RA Applegate (2004) ArticleTitleQuantifying scatter in Shack-Hartmann images to evaluate nuclear cataract J Refract Surg 20 S515–522 Occurrence Handle15523968

    PubMed  Google Scholar 

  18. T Kuroda T Fujikado S Ninomiya N Maeda Y Hirohara T Mihashi (2002) ArticleTitleEffect of aging on ocular light scatter and higher order aberrations J Refract Surg 18 S598–602 Occurrence Handle12361165

    PubMed  Google Scholar 

  19. T Fujikado T Kuroda N Maeda et al. (2004) ArticleTitleLight scattering and optical aberrations as objective parameters to predict visual deterioration in eyes with cataracts J Cataract Refract Surg 30 1198–1208 Occurrence Handle15177593 Occurrence Handle10.1016/j.jcrs.2003.12.023

    Article  PubMed  Google Scholar 

  20. JF Hair RE Anderson RL Tatham WC Black (1998) Multivariate data analysis EditionNumber5th ed Prentice Hall Upper Saddle River, NJ

    Google Scholar 

  21. J Jahns SJ Walker (1990) ArticleTitleTwo-dimensional array of diffractive microlenses fabricated by thin film deposition Appl Opt 7 931–936 Occurrence Handle10.1364/AO.29.000931

    Article  Google Scholar 

  22. B Pierscionek RJ Green SG Dolgobrodov (2002) ArticleTitleRetinal images seen through a cataractous lens modeled as a phase-aberrating screen J Opt Soc Am A Opt Image Sci Vis 19 1491–1500 Occurrence Handle12152689

    PubMed  Google Scholar 

  23. M Born E Wolf (1980) Principles of optics EditionNumber6th ed Pergamon Oxford

    Google Scholar 

  24. P Artal I Iglesias N Lopez-Gil DG Green (1995) ArticleTitleDouble-pass measurements of the retinal-image quality with unequal entrance and exit pupil sizes and the reversibility of the eye's optical system J Opt Soc Am A Opt Image Sci Vis 12 2358–2366 Occurrence Handle7500217 Occurrence Handle1:STN:280:DyaK287it1Sjsw%3D%3D Occurrence Handle10.1364/JOSAA.12.002358

    Article  PubMed  CAS  Google Scholar 

  25. L Diaz-Santana JC Dainty (2001) ArticleTitleEffects of retinal scattering in the ocular double-pass process J Opt Soc Am A Opt Image Sci Vis 18 1437–1444 Occurrence Handle11444533 Occurrence Handle1:STN:280:DC%2BD3MzovFOjsg%3D%3D

    PubMed  CAS  Google Scholar 

  26. CF Bohren DR Huffman (1983) Absorption and scattering of light by small particles Wiley New York

    Google Scholar 

  27. Laven P. MiePlot. http://www.philiplaven.com/mieplot.htm.

  28. TJ van den Berg H Spekreijse (1997) ArticleTitleNear infrared light absorption in the human eye media Vision Res 37 249–253 Occurrence Handle9068825 Occurrence Handle1:STN:280:DyaK2s3itFOkuw%3D%3D Occurrence Handle10.1016/S0042-6989(96)00120-4

    Article  PubMed  CAS  Google Scholar 

  29. KO Gilliland CD Freel S Johnsen W Craig Fowler MJ Costello (2004) ArticleTitleDistribution, spherical structure and predicted Mie scattering of multilamellar bodies in human age-related nuclear cataracts Exp Eye Res 79 563–576 Occurrence Handle15381040 Occurrence Handle1:CAS:528:DC%2BD2cXnslKrt7k%3D Occurrence Handle10.1016/j.exer.2004.05.017

    Article  PubMed  CAS  Google Scholar 

  30. V Tuchin (2000) Tissue optics: light scattering methods and instruments for medical diagnosis SPIE Press Bellingham, Washington

    Google Scholar 

  31. MJ Cox DA Atchison DH Scott (2003) ArticleTitleScatter and its implications for the measurement of optical image quality in human eyes Optom Vis Sci 80 58–68 Occurrence Handle12553545 Occurrence Handle10.1097/00006324-200301000-00009

    Article  PubMed  Google Scholar 

  32. TJ van den Berg H Spekreijse (1999) ArticleTitleLight scattering model for donor lenses as a function of depth Vision Res 39 1437–1445 Occurrence Handle10343812 Occurrence Handle1:STN:280:DyaK1M3nsFarsg%3D%3D Occurrence Handle10.1016/S0042-6989(98)00220-X

    Article  PubMed  CAS  Google Scholar 

  33. HC van de Hulst (1957) Light scattering by small particles Wiley New York

    Google Scholar 

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Authors and Affiliations

  1. Department of Visual Science, Osaka University Graduate School of Medicine, Osaka, Japan

    Toshifumi Mihashi, Yoko Hirohara, Kenichiro Bessho & Takashi Fujikado

  2. Department of Ophthalmology, Osaka University Graduate School of Medicine, Osaka, Japan

    Naoyuki Maeda

  3. Department of Ophthalmology, University of Tsukuba, Tsukuba, Japan

    Tetsuro Oshika

  4. Technical Research Institute, Topcon Corporation, Tokyo, Japan

    Toshifumi Mihashi & Yoko Hirohara

Authors
  1. Toshifumi Mihashi
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  2. Yoko Hirohara
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  3. Kenichiro Bessho
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  4. Naoyuki Maeda
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  5. Tetsuro Oshika
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  6. Takashi Fujikado
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Corresponding author

Correspondence to Takashi Fujikado.

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Mihashi, T., Hirohara, Y., Bessho, K. et al. Intensity Analysis of Hartmann-Shack Images in Cataractous, Keratoconic, and Normal Eyes to Investigate Light Scattering. Jpn J Ophthalmol 50, 323–333 (2006). https://doi.org/10.1007/s10384-006-0336-y

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  • DOI: https://doi.org/10.1007/s10384-006-0336-y

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