Abstract
To assess the role of the red fox (Vulpes vulpes) as a reservoir of leishmaniosis, 92 adult foxes of both genders (58 males and 34 females), shot during the regular hunting seasons in Central Italy, were examined. Blood samples were taken as well as samples from spleen, lymph nodes, and skin to investigate the presence of antibodies against Leishmania infantum and the presence of the parasite DNA in tissues, by means of a species-specific polymerase chain reaction, respectively. All tested sera were negative as well as skin samples. Forty eight animals (52.2%) had leishmania DNA in the lymph nodes and the splenic samples from eight of them (8.7%) scored positive also. The present report would indicate that foxes in an L. infantum medium-endemic area seem negligible reservoir for leishmania infection, even if more than 50% of them bear parasite DNA in their lymphatic tissue.
References
Abranches P, Conceicao-silva FM, Ribeiro MMS, Lopes FJ, Gomes LT (1983) Kala-azar in Portugal IV. The wild reservoir: the isolates of a leishmania from a fox. Trans R Soc Trop Med Hyg 77:420–421
Abranches P, Conceicao-silva FM, Silva-pereira MC (1984) Kala-azar in Portugal. V. The sylvatic cycle in the enzootic endemic focus of Arrabida. J Trop Med Hyg 87:197–200
Beck A, Beck R, Kusak J, Gudan A, Martinkovic F, Artukovic B, Hohsteter M, Huber D, Marinculic A, Grabarevic Z (2008) A case of visceral leishmaniosis in a gray wolf (Canis lupus) from Croatia. J Wildl Dis 44:451–456
Bettini S, Pozio E, Gradoni L (1980) Leishmaniasis in Tuscany (Italy): (II) Leishmania form wild Rodentia and Carnivora in a human and canine leishmaniasis focus. Trans R Soc Trop Med Hyg 74:77–83
Cavallini P, Santini S (1995) Age deterimination in the red fox in the Mediterranean habitat. Zeitschrift für Säugetierkunde 60:136–142
Criado-fornelio A, Gutierrez-garcia R, Rodriguez-caabeiro F, Reus-garcia E, Roldan-soriano MA, Diaz-sanchez MA (2000) A parasitological survey of wild red foxes (Vulpes vulpes) from the province of Guadalajara Spain. Vet Parasitol 92:245–251
De andrade HM, Reis AB, SL Dos santos, Volpini AC, Marques MJ, Romanha AJ (2006) Use of PCR-RFLP to identify Leishmania species in naturally infected dogs. Vet Parasitol 140:231–238
Dipineto L, Manna L, Baiano A, Gala M, Fioretti A, Gravino AE, Menna LF (2007) Presence of Leishmania infantum in red foxes (Vulpes vulpes) in southern Italy. J Wildl Dis 43:518–520
Dujardin JC, Campino L, Canavate C, Dedet JP, Gadoni L, Soteriadou K, Mazeris A, Ozbel Y, Boelaert M (2008) Spread of vector-borne diseases and neglect of leishmaniasis, Europe. Emerg Infect Dis 14 (7)
Fisa R, Gallego M, Castillejo S, Aisa MJ, Serra T, Riera C, Carrio J, Gallego J, Portus M (1999) Epidemiology of canine leishmaniosis in Catalonia (Spain). The example of the Priorat focus. Vet Parasitol 83:87–97
Francino O, Altet L, Sàanchez-Robert E, Rodriguez A, Solano-Gallego I, Albertola J, Ferret L, Sàanchez A, Roura X (2006) Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. Vet Parasitol 137:214–221
Gortazar C (1999) Ecologıa y patologıa del zorro (Vulpes vulpes L.) en el Valle del Ebro. Consejo de Proteccion de la Naturaleza de Aragon (Ed). pp. 180–181
Mancianti F, Meciani N (1988) Specific serodiagnosis of canine leishmaniasis by indirect immunofluorescence, indirect hemagglutination, and counter immunoelectrophoresis. Am J Vet Res 49:1409–1411
Mancianti F, Mignone W, Galastri F (1994) Serologic survey for leishmaniasis in free-living red foxes (Vulpes vulpes) in Italy. J Wildl Dis 30:454–456
Manna L, Vitale F, Reale S, Caracapa S, Pavone LM, Morte RD, Cringoli G, Staino N, Gravino AE (2004) Comparison of different tissue sampling for PCR-based diagnosis and follow-up of canine visceral leishmaniosis. Vet Parasitol 125:251–262
Martin-iniesta F, Martin-iniesta E, Martin-luengo E (1982) Papel de perros y zorros como reservorio de leishmaniosis en la regio´n murciana. Rev Iber Parasitol 42:307–313
Mettler M, Grimm F, Capelli G, Camp H, Deplazes P (2005) Evaluation of enzyme-linked immunosorbent assays, an immunofluorescent-antibody test, and two rapid tests (immunochromatographic-dipstick and gel tests) for serological diagnosis of symptomatic and asymptomatic Leishmania infections in dogs. Jour Microbiol 43:5515–5519
Pedonese F, Mancianti F, Bernardini S, Melosi M (2000) Preliminary records on canine leishmaniasis in the province of Pisa (Tuscany, Italy). Animal Biol 9:101–103
Rioux JA, Albaret JL, Houin R, Dedet JP, Lanotte G (1968) Ecologie des leishmanioses dans le sud de la France. 2. Les reservoirs selvatiques. Infestation spontanee de renard (Vulpes vulpes L.). Annal Parasitol Hum Comp 43:421–428
Rodgers MR, Popper SJ, Wirth DF (1990) Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol 71:267–275
Sastre N, Francino O, Ramirez O, Ensenat C, Sanchez A, Altet A (2008) Detection of Leishmania infantum in captive wolves from Southwestern Europe. Vet Parasitol 158:117–120
Semiao-santos SJ, Abranches P, Silva-pereira MCD, Santos-gomez GM, Fernandes JP, Vetter JCM (1996) Reliability of serological methods for detection of Leishmaniasis in Portuguese domestic and wild reservoirs. Mem Inst Oswaldo Cruz 91:747–750
Sobrino R, Ferroglio E, Oleaga A, Romano A, Millan J, Revilla M, Arnal M, Trisciuoglio MC, Gortazar C (2008) Characterization of widespread canine leishmaniasis among wild carnivores from Spain. Vet Parasitol 155:198–203
Verin R, Mazzoni della stella R, Fratini F, Ebani VV, Macchioni F, Merildi V, Mancianti F, Poli A (2006) Preliminary survey on parasitic and infectious diseases in a red fox (Vulpes vulpes) population living in the central Italy. Proc EWDA Meeting p. 77
Acknowledgments
The authors would like to thank the Province of Pisa and Regione Toscana that made this study possible by providing funding and especially the hunters of the Province of Pisa and their associations (ATC 14 and 15) for their work and their helpfulness. Authors also acknowledge the students of Veterinary Medicine course of the University of Pisa who helped during necropsies and sampling.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by C. Gortázar
Rights and permissions
About this article
Cite this article
Verin, R., Poli, A., Ariti, G. et al. Detection of Leishmania infantum DNA in tissues of free-ranging red foxes (Vulpes vulpes) in Central Italy. Eur J Wildl Res 56, 689–692 (2010). https://doi.org/10.1007/s10344-010-0395-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10344-010-0395-8