Abstract
Forestry practices such as afforestation of former agricultural lands and early forest thinning are applied in several countries. These management strategies increase wood production potential and expand forest areas. However, knowledge of the impact of these practices on the diversity and resilience of soil fungal communities is scarce. This study investigated soil fungal community composition of young (21–40 years-old) Norway spruce (Picea abies (L.) Karst.) dominated stands located in Latvia and Estonia. The study includes data from 62 sampling sites and 2480 soil cores. Fungal internal transcribed spacer amplicons (ITS1-5.8S-ITS2 rDNA region) of DNA extracted from forest floor and fine soil fractions were sequenced using the PacBio sequencing platform. 3176 quality filtered OTUs were detected, and 73.9% of these were identified as fungi. Fungal community composition was mainly differentiated based on soil pH and sampling site. Regarding former land use, relative abundance of the genus Solicoccozyma was higher in samples from former agricultural sites and some species from genera Cortinarius and Russula were identified as more indicative of particular former land use. Litter saprotrophic fungi and fungi of the genus Thelephora were significantly more highly represented in unmanaged sites than in sites where thinning was performed. In conclusion, differences among soil fungal communities are mainly influenced by soil pH and sampling site. Former land use and management have a significant effect on specific genera of ectomycorrhizal and saprotrophic fungi.
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Acknowledgements
This research was funded by European Regional Development Fund's Post-doctoral Research project No. 1.1.1.2/VIAA/2/18/298. Research in Estonian sites was supported by the Norway-Baltic financial mechanism (grant EMP442), Novo Nordisk Fonden (Silva Nova), State Forest Management Centre project T160148 and the Estonian Research Council Grants PSG136 and PRG1615. The authors acknowledge LSFRI Silava colleagues Mudrīte Daugaviete for sharing her object databases, Kaspars Polmanis and Brigita Javoiša for the assistance in field and laboratory work, colleagues from the Forest Environmental laboratory for soil analysis, Dainis Edgars Ruņģis for language revision, and Rasmus Puusepp (University of Tartu) for the assistance in laboratory work.
Funding
This research was funded by European Regional Development Fund's Post-doctoral Research project No. 1.1.1.2/VIAA/2/18/298 “Determining the risk of Heterobasidion root-rot and fungal communities in roots of Norway spruce stands on former agricultural land.” Research in Estonian sites was supported by the Norway-Baltic financial mechanism (grant EMP442), Novo Nordisk Fonden (Silva Nova), State Forest Management Centre project T160148 and the Estonian Research Council Grants PSG136 and PRG1615.
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Darta Klavina contributed to conceptualization, formal analysis, investigation, data curation, visualization, writing—original draft, and funding acquisition. Leho Tedersoo contributed to conceptualization, methodology, investigation, resources, data curation, writing—review and editing, and funding acquisition. Ahto Agan contributed to formal analysis, data curation, and writing—review and editing. Kalev Adamson contributed to investigation and data curation. Kriss Bitenieks contributed to formal analysis, data curation, and writing—review and editing. Talis Gaitnieks contributed to conceptualization and writing—review and editing. Rein Drenkhan contributed to conceptualization, methodology, investigation, writing—review and editing, and funding acquisition.
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Klavina, D., Tedersoo, L., Agan, A. et al. Soil fungal communities in young Norway spruce-dominant stands: footprints of former land use and selective thinning. Eur J Forest Res 141, 503–516 (2022). https://doi.org/10.1007/s10342-022-01454-8
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DOI: https://doi.org/10.1007/s10342-022-01454-8