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Chemical composition, phytotoxic and antifungal activities of Pinus pinea essential oil

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Abstract

The chemical composition of essential oils isolated by hydrodistillation from the aerial parts of Tunisian Pinus pinea Linn. was analyzed by GC and GC/MS. Sixty-six compounds were identified, representing 98.5% of total oil, which was found to be rich in monoterpene hydrocarbons (73.1%) particularly limonene (54.1%), α-pinene (7.7%), and β-pinene (3.4%). The yield and the physico-chemical properties were determined. Results of the antifungal activity study by in vitro contact assay showed that P. pinea oil significantly inhibited the growth of ten plant pathogenic fungi. Moreover, herbicidal properties of the oil, tested on Sinapis arvensis L., Lolium rigidum Gaud., and Raphanus raphanistrum L., indicated that the oil completely inhibited the seed germination at a high concentration, while at low doses the oil acted by decreasing and delaying the germination and inhibiting the seedling growth of all tested weeds unlike the commercial herbicide. Our results showed that P. pinea essential oil could be valorized as bioproduct for biocontrol of weeds and fungal plant diseases.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Département de Biologie, Faculté des Sciences de Bizerte, Université de Carthage, Zarzouna, 7021, Bizerte, Tunisia

    Ismail Amri

  2. Laboratoire d’Ecologie Forestière, Institut National de Recherches en Génie Rural, Eaux et Forêts. BP 10, 2080, Ariana, Tunisia

    Ismail Amri, Lamia Hamrouni & Tarek Fezzani

  3. Laboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hédi Karray, 2080, Ariana, Tunisia

    Samia Gargouri

  4. Laboratoire de Physiologie Moléculaire des Plantes, Centre de Biotechnologie de Borj-Cédria, BP 901, 2050, Hammam-Lif, Tunisia

    Mohsen Hanana

  5. Laboratoire de Chimie, Institut Supérieur d’Education et de Formation Continue, 43 Rue de la Liberté, 2019, Le Bardo, Tunisia

    Bassem Jamoussi

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  1. Ismail Amri
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  2. Samia Gargouri
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  3. Lamia Hamrouni
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  4. Mohsen Hanana
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Correspondence to Mohsen Hanana.

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Communicated by J. Gross.

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Amri, I., Gargouri, S., Hamrouni, L. et al. Chemical composition, phytotoxic and antifungal activities of Pinus pinea essential oil. J Pest Sci 85, 199–207 (2012). https://doi.org/10.1007/s10340-012-0419-0

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  • DOI: https://doi.org/10.1007/s10340-012-0419-0

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