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Insecticidal efficacy of Isaria farinosa in different life stages of Pissodes punctatus (Coleoptera: Curculionidae)

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Abstract

The insecticidal activity of an entomopathogenic fungus, Isaria farinosa on pine bark-weevil (Pissodes punctatus) at different life stages underwent laboratory testing. Larvae, pupae and adults of Pissodes punctatus were exposed to a range of concentrations of fungal conidia in suspension for 5 s; mean mortality and LC50 values were calculated. The results showed I. farinosa can effectively infect larvae, pupae and adult pine bark-weevils, and the highest mean mortalities at each life stage can exceed 88%. The mean mortalities increased with higher concentrations of I. farinosa. Larvae were most susceptible with LC50 1.2 × 106 conidia ml−1 (15 days), pupae (30 days) exhibited the second largest effects, and adults (15 days) with LC50 1.72 × 106 and 1.99 × 106 conidia ml−1, respectively, at end of the experiments. This suggests that I. farinosa could be a potential bio-control agent against the pine bark-weevil.

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Acknowledgments

We wish to express our sincere thanks to Mr. Duan Zhaoyao for rearing experimental insects, and as well to Ms. Hong Yingdi for isolation and culturing of fungus strain tested in this study. Many thanks to Ms. Even Pay for editing of manuscript. Our work is supported by Yunnan Provincial Key Technology Research and Development Grant (No. 2003NG12).

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Correspondence to Rongping Kuang.

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Communicated by A. J. Storer.

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Yang, S., Zhuang, H., Li, Y. et al. Insecticidal efficacy of Isaria farinosa in different life stages of Pissodes punctatus (Coleoptera: Curculionidae). J Pest Sci 82, 321–325 (2009). https://doi.org/10.1007/s10340-009-0256-y

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