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Method Development for Determination of Antibiotic Drugs Using Newly Prepared p-Morpholinomethylcalix[4]arene Mesoporous Silica-Based HPLC Column

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Abstract

Mesoporous materials are described by their relatively high surface areas and pore volumes. They possess uniform channels within nanometer range. These materials have numerous applications in catalysis, separation and many other fields. The qualitative and quantitative determination of antibiotic drugs, i.e., ciprofloxacin and cefixime has clinical and analytical importance due to their broad spectrum of antimicrobial activity and stability. Both antibiotic drugs are orally active and have excellent activity against different pathogens. It is for the first time that we have developed an analytical method for the simultaneous analyses of both drugs using a newly developed p-morpholinomethylcalix[4]arene (p-MC4) mesoporous silica-based HPLC column (15 × 3 mm I.D.). Furthermore, separation of these two components was carried out using isocratic elution of methanol and 0.1% aqueous formic acid (70:30 v/v) with flow rate of 1 ml min−1 at retention time of 2.71 and 4.21 min and retention factor 1.85 and 1.19 for ciprofloxacin and cefixime, respectively; while total run time was 5 min. The developed method was repeatable with a relative standard deviation (RSD) of 0.90–2.08% for antibiotic drugs. The limits of detection and quantification of ciprofloxacin and cefixime were obtained within the range of 0.152–0.801 and 0.40–1.23 µg mL−1, respectively. The method is highly applicable, rapid, simple, very reproducible and accurate for the separation and determination of antibiotic drugs.

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Acknowledgements

The authors express gratitude to the National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan for supporting through financial support and providing the compulsory facilities.

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Authors and Affiliations

  1. National Centre of Excellence in Analytical Chemistry, University of Sindh Jamshoro, Jamshoro, Sindh, 76080, Pakistan

    Naeem A. Memon, Ayaz A. Memon, Sayed Tufail Hussain Sherazi, Shahabuddin Memon & Muhammad K. Leghari

  2. Institute of Chemistry, Shah Abdul Latif University, Khairpur Mirs, Sindh, 66020, Pakistan

    Fakhar N. Memon

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  1. Naeem A. Memon
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  2. Ayaz A. Memon
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  3. Fakhar N. Memon
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  4. Sayed Tufail Hussain Sherazi
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Correspondence to Fakhar N. Memon.

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Memon, N.A., Memon, A.A., Memon, F.N. et al. Method Development for Determination of Antibiotic Drugs Using Newly Prepared p-Morpholinomethylcalix[4]arene Mesoporous Silica-Based HPLC Column. Chromatographia 81, 1373–1380 (2018). https://doi.org/10.1007/s10337-018-3580-7

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  • DOI: https://doi.org/10.1007/s10337-018-3580-7

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