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System Maps for Retention of Small Neutral Compounds on a Superficially Porous Ethyl-Bridged, Octadecylsiloxane-Bonded Silica Stationary Phase in Reversed-Phase Liquid Chromatography

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Abstract

The system constants of the solvation parameter model are used to prepare system maps for the retention of small neutral compounds on an ethyl-bridged, ocatadecylsiloxane-bonded superficially porous silica stationary phase (Kinetex EVO C18) for aqueous mobile phases containing 10–70% (v/v) methanol or acetonitrile. Electrostatic interactions (cation-exchange) are important for the retention of weak bases with acetonitrile–water but not methanol–water mobile phase compositions. Compared with a superficially porous octadecylsiloxane-bonded silica stationary phase (Kinetex C18) with a similar morphology but different topology statistically significant differences in selectivity at the 95% confidence level are observed for neutral compounds that vary by size and hydrogen-bond basicity with other intermolecular interactions roughly similar. These selectivity differences are dampened with acetonitrile–water mobile phases, but are significant for methanol–water mobile phase compositions containing <30% (v/v) methanol. A comparison of a totally porous ethyl-bridged, octadecylsiloxane-bonded silica stationary phase (XBridge C18) with Kinetex EVO C18 indicated that they are effectively selectivity equivalent.

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Acknowledgements

Antonia Napoli (Phenomenex) is acknowledged for providing the columns used in this study.

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Authors and Affiliations

  1. CanAm Bioresearch Inc., Winnipeg, MB, R3T 0P4, Canada

    Sanka N. Atapattu

  2. Department of Chemistry, Wayne State University, Detroit, MI, 48202, USA

    Colin F. Poole

  3. Phenomenex Inc., Torrance, CA, 90501, USA

    Mike B. Praseuth

Authors
  1. Sanka N. Atapattu
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  2. Colin F. Poole
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  3. Mike B. Praseuth
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Correspondence to Colin F. Poole.

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Conflict of interest

Authors Atapattu and Poole have no conflict of interest. Author Praseuth is an employee of Phenomenex who manufactured the column used in this study. Authors Atapattu and Poole received no financial support from Phenomenex for this study.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Atapattu, S.N., Poole, C.F. & Praseuth, M.B. System Maps for Retention of Small Neutral Compounds on a Superficially Porous Ethyl-Bridged, Octadecylsiloxane-Bonded Silica Stationary Phase in Reversed-Phase Liquid Chromatography. Chromatographia 80, 1279–1286 (2017). https://doi.org/10.1007/s10337-017-3350-y

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  • DOI: https://doi.org/10.1007/s10337-017-3350-y

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