Abstract
A novel scalable procedure for the thermally initiated polymerisation of bonded monolithic porous layers of controlled thickness within open tubular fused silica capillaries (monoPLOT columns) is presented. Porous polymer layers of either polystyrene-divinylbenzene or butyl methacrylate-ethylene dimethacrylate, of variable thickness and morphology were polymerised inside fused silica capillaries utilising combined thermal initiation and laminar flow of the polymerisation mixture. The procedure enables the production through thermal initiation of monoPLOT columns of varying length, internal diameter, user defined morphology and layer thickness for potential use in both liquid and gas chromatography. The morphology and thickness of the bonded polymer layer on the capillary wall is strongly dependent on the laminar flow properties of the polymerisation mixture and the changing shear stress within the fluid across the inner diameter of the open capillary. Owing to the highly controlled rate of polymerisation and its dependence on fluid shear stress at the capillary wall, the procedure was demonstrably scalable, as illustrated by the polymerisation of identical layers within different capillary diameters.
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References
Golay MJE (1958) Gas chromatography. Academic Press, New York
de Zeeuw J (2011) LC-GC Eur 24:38–45
Collins D, Nesterenko E, Brabazon D, Paull B (2012) Anal Chem 84:3465–3472
Nischang I, Brueggemann O, Svec F (2010) Anal Bioanal Chem 397:953–960
Luo Q, Rejtar T, Wu SL, Karger B (2009) J Chromatogr A 1216:1223–1231
Huang X, Zhang J, Horvath C (1999) J Chromatogr A 858:91–101
Tan ZJ, Remcho VT (1998) J Microcolumn Sep 10(1):99–105
Tan ZJ, Remcho VT (1997) Anal Chem 69(4):581–586
Chuang SC, Chang CY, Liu CY (2004) J Chromatogr A 1044:229–236
Shen TC (1992) J Chromatogr Sci 30(6):239–240
Banu I, Bildea S, Bozga G, Puaux JP (2009) Stud Univ Babes-Bolyai Chem 54(1):227–241
Castro JM, Lipshitz SD, Macosko CW (1982) AIChE J 28(6):973–980
Wehner JF (1978) Chem React Eng Houst. doi:10.1021/bk-1978-0065.ch012
Nesterenko EP, Nesterenko PN, Connolly D, Lacroix F, Paull B (2010) J Chromatogr A 1217:2138–2146
Collins DA, Nesterenko EP, Connolly D, Vasquez M, Macka M, Brabazon D, Paull B (2011) Anal Chem 83(11):4307–4313
Cifuentes A, Diez-Masa JC, Fritz J et al (1998) Anal Chem 70(16):3458–3462
Fox R, McDonald A, Pritchard P (2009) Introduction to fluid mechanics, 7th edn. Wiley, New York
http://www.polymicro.com. Accessed November 2012
Odian G (1981) Principles of polymerization, 2nd edn. Wiley, New York
Acknowledgments
The authors would like to acknowledge financial support from Science Foundation Ireland for the Irish Separation Science Cluster award (Grant No. 08/SRC/B1412).
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The authors declare no competing financial interest.
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Collins, D.A., Nesterenko, E.P., Brabazon, D. et al. Fabrication of Bonded Monolithic Porous Layer Open Tubular (monoPLOT) Columns in Wide Bore Capillary by Laminar Flow Thermal Initiation. Chromatographia 76, 581–589 (2013). https://doi.org/10.1007/s10337-013-2447-1
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DOI: https://doi.org/10.1007/s10337-013-2447-1