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Azithromycin for Enantioseparation of Tetrahydrozoline in NACE

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Abstract

Successful use of macrolide antibiotic azithromycin for enantioseparation of tetrahydrozoline is described in this paper. The effect of experimental parameters such as background electrolyte composition, solvent type, chiral selector concentration, temperature, and applied voltage on the enantioseparation of tetrahydrozoline was studied. The procedure was proposed for the analysis of tetrahydrozoline in pharmaceuticals. Linearity was achieved in the concentration range 5 × 10−2–1 mg mL−1. The azithromycin stability in the background electrolyte and the antibiotic adsorption on the fused-silica capillary were studied. Best enantioseparation with resolution factor 1.6 was achieved in less than 10 min.

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Acknowledgments

This work was supported by the Russian Foundation for Basic Research (Grants No. 12-03-00405-a and No. 12-03-31255-мол-а).

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Authors and Affiliations

  1. Chemistry Department, Lomonosov Moscow State University, 119991, Leninskie Gory, dom.1, str.3, Moscow, Russian Federation

    Margarita V. Lebedeva, Galina A. Bulgakova, Aleksandra F. Prokhorova, Elena N. Shapovalova & Oleg A. Shpigun

  2. Drugs Technology, Khimki, Russian Federation

    Mikhail G. Chernobrovkin

Authors
  1. Margarita V. Lebedeva
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  2. Galina A. Bulgakova
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  3. Aleksandra F. Prokhorova
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  4. Elena N. Shapovalova
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  5. Mikhail G. Chernobrovkin
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  6. Oleg A. Shpigun
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Corresponding author

Correspondence to Margarita V. Lebedeva.

Additional information

Published in the special paper collection Advances in Chromatography and Electrophoresis & Chiranal 2012 with guest editor Jan Petr.

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Lebedeva, M.V., Bulgakova, G.A., Prokhorova, A.F. et al. Azithromycin for Enantioseparation of Tetrahydrozoline in NACE. Chromatographia 76, 375–379 (2013). https://doi.org/10.1007/s10337-012-2347-9

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  • DOI: https://doi.org/10.1007/s10337-012-2347-9

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