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Epitopic Peptides Identified by LC–ELISA and LC–MS

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Abstract

A new strategy of a liquid chromatography (LC)-enzyme linked immunosorbent assay (ELISA) combined with liquid chromatography-mass spectrometry (LC–MS) was developed to identify the epitopic peptides for the antigen in Streptococcus suis. Metallendopeptidase, a potential antigen for S. suis, was digested by trypsin. The tryptic solution was separated by LC under optimal condition and collected into tubes. After it was dried using a cooling vacuum, the immunogenecity of peptides in each tube was investigated by ELISA against its corresponding antibody. All peptides in each tube with positive signals based on ELISA results were identified by LC–MS. Accordingly, six putative peptides of metallendopeptidase were found and synthesized, all of which possessed high antigenicity recognized by the antibody.

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Acknowledgments

Thanks to the Medical Chemistry and Molecular Diagnosis Laboratory of the Hebei University for the FTICR mass spectrometry. The project was supported by Key Laboratory of Pathogenic Microbiology and Immunology, Chinese Academy of Sciences (2009CASPMI-007).

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Authors and Affiliations

  1. Beijing Institute of Genomics, Chinese Academy of Sciences, No. 7 Beitucheng West Road, Chaoyang District, Beijing, 100029, People’s Republic of China

    Chuanqi Zhou

  2. School of Life Science and Technology, Changchun University of Science and Technology, 7089 Weixing Road, Changchun, Jilin, 130022, People’s Republic of China

    Qingshuang Wang

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  1. Chuanqi Zhou
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  2. Qingshuang Wang
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Correspondence to Chuanqi Zhou.

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Zhou, C., Wang, Q. Epitopic Peptides Identified by LC–ELISA and LC–MS. Chromatographia 73, 879–887 (2011). https://doi.org/10.1007/s10337-011-1988-4

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  • DOI: https://doi.org/10.1007/s10337-011-1988-4

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