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Reverse Homogeneous Liquid–Liquid Extraction as a Miniaturized Method for Extraction of Aflatoxins from Pistachio and Wheat and LC Post-column Derivatization-Fluorescence Detection

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Abstract

A rapid and simple quantitative method for the simultaneous determination of the four aflatoxins (AFs) B1, B2, G1 and G2 was developed using reverse homogeneous liquid–liquid extraction (RHLEE) and HPLC post-column derivatization-fluorescence (HPLC-FL) detection. The method based on the rapid extraction of AFs from a methanolic sample into chloroform after addition of water containing KBr, with a method we called reverse homogeneous liquid–liquid extraction. Recoveries were in the range of 88–125% and the limits of detection were between 0.001–0.042 ng g−1 for different AFs. Wheat and pistachio were chosen for the analysis of real samples and the method was also successfully applied to a FAPAS® TEST MATERIAL (T04151).

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Authors and Affiliations

  1. Department of Toxicology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Jalal Hassan

  2. International Goods Inspection Company, Tehran, Iran

    Shahoo Habibi

Authors
  1. Jalal Hassan
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  2. Shahoo Habibi
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Correspondence to Jalal Hassan.

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Hassan, J., Habibi, S. Reverse Homogeneous Liquid–Liquid Extraction as a Miniaturized Method for Extraction of Aflatoxins from Pistachio and Wheat and LC Post-column Derivatization-Fluorescence Detection. Chromatographia 73, 1005–1008 (2011). https://doi.org/10.1007/s10337-011-1981-y

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  • DOI: https://doi.org/10.1007/s10337-011-1981-y

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