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Determination of T-2 and HT-2 Toxins in Traditional Chinese Medicine Marketed in China by LC–ELSD after Sample Clean-Up by Two Solid-Phase Extractions

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Abstract

LC–ELSD has been applied for the determination of T-2 and HT-2 toxins in Traditional Chinese Medicine (TCM) marketed in China. For sample preparation, two clean-up procedures were developed using Florisil and MycoSep227 for solid-phase extraction. Limits of detection (10 ng g−1) as well as recoveries are described. A total of 138 samples of 46 commercially available TCM samples was analyzed; only one sample was determined to contain T-2 at a level of 64.0 ng g−1, and positive results were further confirmed by LC–ESI–MS–MS.

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Acknowledgments

We thank China National Group Corporation of Traditional & Herbal Medicine for encouraging us to carry on with our survey. Also, we are grateful to Yantao Yue and Meihua Yang for technical support. This work was supported by Fundamental Scientific Research Project of China (YZ-1-20) and Scientific Research Project of Traditional Chinese Medicine Vocation in 2008 (200807042).

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Authors and Affiliations

  1. Hainan Provincial Key Laboratory of R&D of Tropical Herbs, Hainan Medical College, 571101, Hainan, China

    Yinfeng Tan

  2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, 100193, Beijing, China

    Ying Kuang & Jianwei Wu

  3. Technology Development Center, China National Group Corp. of Traditional & Herbal Medicine, 102600, Beijing, China

    Runhuai Zhao, Bo Chen & Jianwei Wu

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  1. Yinfeng Tan
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  2. Ying Kuang
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  3. Runhuai Zhao
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  4. Bo Chen
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  5. Jianwei Wu
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Correspondence to Jianwei Wu.

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Tan, Y., Kuang, Y., Zhao, R. et al. Determination of T-2 and HT-2 Toxins in Traditional Chinese Medicine Marketed in China by LC–ELSD after Sample Clean-Up by Two Solid-Phase Extractions. Chromatographia 73, 407–410 (2011). https://doi.org/10.1007/s10337-010-1890-5

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  • DOI: https://doi.org/10.1007/s10337-010-1890-5

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