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Simultaneous Determination of Four Sudan Dyes in Egg Yolks by Molecularly Imprinted SPE Coupled with LC-UV Detection

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Abstract

A highly selective molecularly imprinted solid-phase extraction (MISPE) combined with liquid chromatography-ultraviolet detection was developed for the simultaneous isolation and determination of four Sudan dyes (I, II, III and IV) in egg-yolk products. The imprinted microspheres synthesized by suspension polymerization using phenylamine–naphthol as mimic template show high selectivity and affinity to the four kinds of Sudan dyes and were successfully applied as selective sorbents of solid-phase extraction for the simultaneous determination of the four Sudans from egg-yolk samples. Good linearity was obtained in a range of 0.062–10 μg g−1 and the average recoveries of the four Sudans at three spiked levels ranged from 94.1 to 102.5% with the relative standard deviations less than 5.8%. The developed extraction protocol eliminated the effect of template leakage on quantitative analysis and could be applied for the determination of Sudans in complicated food samples.

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References

  1. Rebane R, Leito I, Yurchenko S, Herodes K (2010) J Chromatogr A 1217:2747–2757

    Article  CAS  Google Scholar 

  2. Golka K, Kopps S, Myslak ZW (2004) Toxicol Lett 151:203–210

    Article  CAS  Google Scholar 

  3. Ahlstrom LH, Eskilsson CS, Bjorklund E (2005) Trends Anal Chem 24:49–56

    Article  CAS  Google Scholar 

  4. Calbiani F, Careri M, Elviri L, Mangia A, Pistara L, Zagnoni I (2004) J Chromatogr A 1042:123–130

    Article  CAS  Google Scholar 

  5. Cui X, Chu X, Jerry AZ, Fang Y, Yong W, Ling Y (2010) Chromatographia 71:139–142

    Article  CAS  Google Scholar 

  6. He L, Su Y, fang B, Shen X, Zeng Z, Liu Y (2007) Anal Chim Acta 594:139–146

    Article  CAS  Google Scholar 

  7. Wu YL, Li C, Xia X, Liu YJ, Shen JZ (2010) J Chromatogr Sci 48:63–67

    Google Scholar 

  8. Cornet V, Govaert Y, Moens G, Van LJ, Degroodt M (2006) J Agric Food Chem 54:639–644

    Article  CAS  Google Scholar 

  9. Ertaşa E, Özera H, Alasalvar C (2007) Food Chem 105:756–760

    Article  Google Scholar 

  10. Mejia E, Ding Y, Mora MF, Garcia CD (2007) Food Chem 102:1027–1033

    Article  CAS  Google Scholar 

  11. Chailapakul O, Wonsawat W, Siangproh W, Grudpan K, Zhao Y, Zhu Z (2008) Food Chem 109:876–882

    Article  CAS  Google Scholar 

  12. Anfossi L, Baggiani C, Giovannoli C, Giraudi G (2009) Food Addit 26:800–807

    Article  CAS  Google Scholar 

  13. Anibal CVD, Odena M, Ruisánchez I, Callao MP (2009) Talanta 79:887–892

    Article  Google Scholar 

  14. Liu Y, Song Z, Dong F, Zhang L (2007) J Agric Food Chem 55:614–617

    Article  CAS  Google Scholar 

  15. Gao XF, Liu HY, Song ZH, He XL, Dong FX (2007) Spectroscopy 21:135–141

    CAS  Google Scholar 

  16. López-Jiménez FJ, Rubio S, Pérez-Bendito D (2010) Food Chem 121:763–769

    Article  Google Scholar 

  17. Habibi MH, Hassanzadeh A, Zeini-Isfahani A (2006) Dyes Pigments 69:93–101

    Article  CAS  Google Scholar 

  18. Wu Y (2010) Food Chem 121:580–584

    Article  CAS  Google Scholar 

  19. Pardoa O, Yusà V, León N, Pastor A (2009) Talanta 78:178–186

    Article  Google Scholar 

  20. Murty MRVS, Chary NS, Prabhakar S, Raju NP, Vairamani M (2009) Food Chem 115:1556–1562

    Article  CAS  Google Scholar 

  21. Zhang Y, Zhang Z, Sun Y (2006) J Chromatogr A 1129:34–40

    Article  CAS  Google Scholar 

  22. Xu Z, Wang S, Fang G, Song J, Zhang Y (2010) Chromatographia 71:397–403

    Article  CAS  Google Scholar 

  23. Arce L, Nozal L, Simonet BM, Valcárcel M, Ríos A (2009) Trends Anal Chem 28:842–853

    Article  CAS  Google Scholar 

  24. Liu W, Zhao WJ, Chen JB, Yang MM (2007) Anal Chim Acta 605:41–45

    Article  CAS  Google Scholar 

  25. Yu C, Liu Q, Lan L, Hu B (2008) J Chromatogr A 1188:124–131

    Article  CAS  Google Scholar 

  26. Fan Y, Chen M, Shentu C, El-Sepai F, Wang K, Zhu Y, Ye M (2009) Anal Chim Acta 650:65–69

    Article  CAS  Google Scholar 

  27. Mahony JO, Nolan K, Smyth MR, Mizaikoff B (2005) Anal Chim Acta 534:31–39

    Article  CAS  Google Scholar 

  28. Qiao F, Sun H, Yan H, Row KH (2006) Chromatographia 64:625–634

    Article  CAS  Google Scholar 

  29. Zhang Z, Zhang H, Hu Y, Yao S (2010) Anal Chim Acta 661:173–180

    Article  CAS  Google Scholar 

  30. Wang S, Xu Z, Fang G, Duan Z, Zhang Y, Chen S (2007) J Agric Food Chem 10:3869–3876

    Article  Google Scholar 

  31. Puoci F, Garre C, Iemma F, Muzzalupo R, Spizzirri UG, Picci N (2005) Food Chem 93:349–353

    Article  CAS  Google Scholar 

  32. Baggiani C, Anfossi L, Baravalle P, Giovannoli C, Giraudi G, Barolo C, Viscardi G (2009) J Sep Sci 32:3292–3300

    Article  CAS  Google Scholar 

  33. Hou X, Li Y, Cao S, Zhang Z, Wu Y (2010) Chromatographia 71:135–138

    Article  CAS  Google Scholar 

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Acknowledgments

The project was sponsored by the National Natural Science Foundation of China (20905019, 21011140338), the Natural Science Foundation of Hebei (B2010000209) and the Natural Science Foundation of Hebei University (y2008137).

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Authors and Affiliations

  1. Key Laboratory of Pharmaceutical Quality Control of Hebei Province and College of Pharmacy, Hebei University, Baoding, 071002, China

    Jindong Qiao, Hongyuan Yan, Hui Wang, Yupei Wu, Pengyu Pan & Yuru Geng

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  1. Jindong Qiao
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  2. Hongyuan Yan
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  3. Hui Wang
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  4. Yupei Wu
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  5. Pengyu Pan
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Correspondence to Hongyuan Yan.

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Qiao, J., Yan, H., Wang, H. et al. Simultaneous Determination of Four Sudan Dyes in Egg Yolks by Molecularly Imprinted SPE Coupled with LC-UV Detection. Chromatographia 73, 227–233 (2011). https://doi.org/10.1007/s10337-010-1872-7

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  • DOI: https://doi.org/10.1007/s10337-010-1872-7

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