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An oxygen-consuming phantom simulating perfused tissue to explore oxygen dynamics and 19F MRI oximetry

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Abstract

Objective

This study presents a reproducible phantom which mimics oxygen-consuming tissue and can be used for the validation of 19F MRI oximetry.

Materials and methods

The phantom consists of a haemodialysis filter of which the outer compartment is filled with a gelatin matrix containing viable yeast cells. Perfluorocarbon emulsions can be added to the gelatin matrix to simulate sequestered perfluorocarbons. A blood-substituting perfluorocarbon fluid is pumped through the lumen of the fibres in the filter. 19F relaxometry MRI is performed with a fast 2D Look-Locker imaging sequence on a clinical 3T scanner.

Results

Acute and perfusion-related hypoxia were simulated and imaged spatially and temporally using the phantom.

Conclusions

The presented experimental setup can be used to simulate oxygen consumption by somatic cells in vivo and for validating computational biophysical models of hypoxia, as measured with 19F MRI oximetry.

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Authors and Affiliations

  1. Department of Radiation Oncology and Experimental Cancer Research, Ghent University, De Pintelaan 185, 9000, Gent, Belgium

    Steven H(ubert) Baete, Jan Vandecasteele, Wilfried De Neve & Yves De Deene

  2. MEDISIP-IBBT, Ghent University, De Pintelaan 185, 9000, Gent, Belgium

    Steven H(ubert) Baete & Yves De Deene

  3. Faculty of Applied Engineering Sciences, University College Ghent, Schoonmeersstraat 52, 9000, Gent, Belgium

    Luc Colman

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  1. Steven H(ubert) Baete
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  2. Jan Vandecasteele
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  3. Luc Colman
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  4. Wilfried De Neve
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  5. Yves De Deene
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Correspondence to Steven H(ubert) Baete.

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Baete, S.H., Vandecasteele, J., Colman, L. et al. An oxygen-consuming phantom simulating perfused tissue to explore oxygen dynamics and 19F MRI oximetry. Magn Reson Mater Phy 23, 217–226 (2010). https://doi.org/10.1007/s10334-010-0219-6

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  • DOI: https://doi.org/10.1007/s10334-010-0219-6

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